LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION – BIO TECHNOLOGY
SECOND SEMESTER – April 2009
BT 2813 – GENETIC ENGINEERING
Date & Time: 20/04/2009 / 1:00 – 4:00 Dept. No. Max. : 100 Marks
SECTION – A
Answer all the questions (20 marks)
- Choose the best answer. (5 X 1 = 5)
- Which of the following is not an example of DNA cleaving reagent used in
- a) DNAse I b) Hydroxyl radical c) Dimethyl sulfate d) β-mercaptoethanol
- The pore size for the filters used in dot blots for large nucleic acids is ____ µm.
- a) 0.25 b) 0.35 c) 0.45 d) 0.55
- Which is the optimum reaction temperature for Taq 1 enzyme
- a) 25 degrees b) 37 degrees c) 65 degrees d) 45 degrees
- Which is the first licensed drug produced through rDNA technology?
- a) Human insulin b) Hepatitis-B vaccine c) Somatostatin d)tPA
- Alpha complementation is used in
- a) bacterial transformation b) transfection c) transduction d) conjugation
- Say True or False; if false, state reasons. (5 X 1 = 5)
- Polylinkers are also called as Multiple Cloning Sites.
- Science published Taq polymerase as the molecule of the year in 1989.
- Enhancers are bidirectional gene regulatory elements.
- EMBL3 is used for the construction of cDNA libraries.
- Agrobacterium transformation is mainly used for monocots.
III. Complete the following. (5 X 1 = 5)
- ________ and ________ discovered the concept of RNAi.
- IPTG stands for _________.
- ________ is called as molecular glue.
- ‘aad’ confers resistance to _______ and ________ antibiotics.
- ________ radioactive isotope is used for in situ hybridization.
- Answer the following in one or two sentences, each in about 50 words. (5 X 1 =5)
- Site-directed mutagenesis
SECTION – B
- Answer any five questions, each in about 350 words only. (5 X 8 = 40)
- What are the features of molecular probes? Differentiate between radioactive and
non-radioactive methods of labeling.
- List out the components of hybridization buffer and add a note on stringency control.
Add a note on autoradiography.
- Mention the parameters to be considered for primer designing for PCR applications.
How do you design primers using Primer3 software?
- Differentiate RFLP and RAPD. How can the former technique be used for detection
of genetic disorders and epidemiological typing?
- Enumerate the steps involved in bacterial transformation.
- Give an account on subtractive hybridization with an example.
- What is electroporation? Mention its advantages and disadvantages.
- What are the advantages of organelle transformation and give a brief description of it.
SECTION – C
- Answer the following, each in about 1500 words. (2 x 20 = 40)
- a) ‘The nub of the new (genetic engineering) technology is to move genes back and forth, not only across species lines, but across any boundaries that divide living organisms. The results will be essentially new organisms, self-perpetuating and hence permanent. Once created, they cannot be recalled’ – Discuss this statement of George Wald, throwing light upon the technical, ethical, social, ecological and legal issues involved in Genetic Engineering. (3 + 3 + 3 + 5 + 6)
- b) Explain the post-transcriptional gene silencing machinery using RNA interference technology. Differentiate between siRNA and miRNA, and add a note on the various applications of RNAi. (12 + 3 + 5)
- a) How do you generate a transgenic tobacco plant resistant to TMV (Tobacco
Mosaic Virus)? Describe the procedure with appropriate diagram.
- b) Enlist the characteristics of various yeast vectors used for transformation, and also mention their merits and demerits.
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