Loyola College B.Sc. Plant Biology and Biotechnology April 2006 Fermentation Technology Question Paper PDF Download

             LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & BIOTECHNOLOGY

IB 22

SIXTH SEMESTER – APRIL 2006

                                               PB 6606 – FERMENTATION TECHNOLOGY

(Also equivalent to PB 6601)

 

 

Date & Time : 24-04-2006/9.00-12.00         Dept. No.                                                       Max. : 100 Marks

 

 

Part A

Answer all questions                                                                         (20 Marks)

 

I  Choose the best answer                                                                               (5 x 1 = 5)

 

  1. ______ involves prevention of escape of viable cells from a fermenter.
  2. Containment b. Asepsis c. Contention  d. Sterilisation
  3. Growth of filamentous cultures of fermentation is achieved by
  4. deep-jet fermenters b. the packed tower
  5. air-lift fermenters d. the cyclone column
  6. ______ sensor is an integral part of the fermenter and the sensed values sent directly for process control.
  7. On-line b. Off-line c. Biosensor     d. In-line
  8. Which phase is equivalent to the log phase of microbial growth kinetics?
  9. Trophophase b. Idiophase c. Intermediary phase  d. Balanced phase
  10. ______ is exclusively used in continuous culture fermentation.
  11. Turbidostat b. Chemostat c. Biostat         d. Multistage fermenter.

 

  1. State whether the following are true or false (5 x 1 = 5)

 

  1. In the context of industrial microbiologists, fermentation is a process of product formation by the mass culture of a microorganism.
  2. Non-mutant isolates produce very high concentrations of commercially important products.
  3. In biosensors, the transducer is a biological component.
  4. EDTA acts as a chelator in the fermentation media.
  5. Variable area meter is used for sensing the rate of stirring.

 

III. Complete the following                                                                            (5 x 1 = 5)

 

  1. A fermenter made of a glass cylinder with stainless steel top and bottom plates could be sterilized ______.
  2. ______ are ancillary equipments used for controlling the liquid and gas flows in fermenters.
  3. In microbial growth kinetics, the balanced phase is equivalent to __________.
  4. Carbon and energy source + Nitrogen source + O2 + other requirements ® _______ + products + CO2 +  H2O + D.
  5. To measure and to control dissolved oxygen ______ electrodes are used in pilot and production fermenters.

 

 

 

 

 

  1. Answer the following in one or two sentences (5 x 1 = 5)

 

  1. Define fermentation in biochemical sense.
  2. Identify the components of a control loop.
  3. Enlist the antifoam devices.
  4. Distinguish between trophophase and idiophase
  5. What are transformation processes?

 

Part B

 

Answer any five of the following, each not exceeding 350 words                      (5 x 8 = 40)

 

  1. Enumerate the range of fermentation processes.
  2. Explain the methods measuring the following process variables during fermentation:
  3. flow measurement b. foam sensing
    c. microbial biomass   d. dissolved oxygen
  4. Give a brief account of media preparation for industrial fermentation.
  5. Describe the working mechanism of different types of biosensors.
  6. Work out the kinetics of microbial growth during continuous culture fermentation.
  7. Comment on the following fermentation vessels:
  8. Waldhof – type fermenter b. The tower fermenter
  9. Cylindro – conical vessels d. Air – lift fermenters
  10. Bring out the considerations towards design and construction of a fermenter.
  11. How the control systems operate on various process variables during fermentation?

 

Part C

 

Answer the following, each not exceeding 1500 words.                   (2 x 20 = 40)

 

29 a. Trace the chronological development of the fermentation industry.

(or)

  1. Explain the application of computers towards fermentation technology.

 

  • Discuss how chromatographic techniques are applied towards product recovery and purification in fermentation industries.

(or)

  1. How to isolate, preserve and to improve the industrially important microorganisms?

 

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Loyola College B.Sc. Plant Biology and Biotechnology April 2007 Fermentation Technology Question Paper PDF Download

LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

B.Sc.  DEGREE EXAMINATION –PLANT BIOLOGY & PLANT BIO-TECH.

IB 23

SIXTH SEMESTER – APRIL 2007

PB 6606FERMENTATION TECHNOLOGY

 

 

Date & Time: 20/04/2007 / 9:00 – 12:00       Dept. No.                                                                 Max. : 100 Marks

 

 

Part A

Answer all questions

 

  1. Choose the best answer (5 x 1 = 5)
  2. Microbe mediated conversion of a compound into a structurally related one is called
  3. microbial transformation b. compound transformation
  4. biotransformation d. molecular transformation

 

  1. ____ = μmaxs / (Ks + s)
  2. ½ μ b. ½ μmax c. μ2     d. μ

 

  1. ______ is required to achieve a number of mixing objectives during fermentation.
  2. Agitator b. Impeller c. Agitator or impeller d. Acetator

 

  1. Measurement and control of dissolved oxygen is accomplished using _____ electrodes.
  2. polarographic b. reference c. Bourdon tube          d. tubing

 

  1. Reversible exchange of ions between a liquid phase and a solid phase is referred to as
  2. ion-exchange b. ion-transfer c. ion-reversion            d. ion-mobilisation

 

  1. State whether the following statements are true or false (5 x 1 = 5)
  2. Baffles are incorporated into the fermenter to prevent vortex and to improve aeration efficiency.
  3. In continuous culture systems, D = F / W.
  4. During fermentation, monitoring equipment produces information to indicate the progress of the process.
  5. In computer administered fermentation, data reduction is performed by the data analysis system.

 

III. Complete the following                                                                          (5 x 1 =5)

  1. ______ technology has revolutionised and extended the production of several potential fermentation products.

 

  1. In microbial growth kinetics, the maintenance phase is equivalent to _______ phase.

 

  1. ________ fermenters are used highly for the production of Single Cell Proteins.

 

  1. Carbon & Energy source + Nitrogen source + O2 + other requirements → Biomass + _____ + CO2 + H2O + ∆.

 

  1. ________ sensor is not an integral part of the fermenter.

 

 

 

  1. Answer the following, each within 50 words (5 x 1 = 5)
  2. Define fermentation in strict biochemical sense.
  3. What is fed – batch culture?
  4. Distinguish between asepsis and containment.
  5. Functionally differentiate In-line and On-line sensors.
  6. What are antifoams?

Part B

 

Answer any five of the following, each within 350 words                                                 (5 x 8 = 40)

  1. Comment on the range of fermentation process.
  2. Enumerate the basic functions of a fermenter.
  3. Describe in brief the methods of preservation of industrially important microorganisms.
  4. Give an account of the control systems of process variables in fermentation process.
  5. Derive for the kinetics of batch fermentation.
  6. Give the schematic representation of down stream processing with a brief explanation.
  7. Write down the various applications of Biosensors.
  8. Comment on the following fermentation vessels:
  9. Waldhof – type fermenter ii.  Tower fermenter

iii. Cylindro – Conical vessels                   iv. Air – lift fermenters

 

Part C

 

Answer the following, each within 1500 words. Draw necessary diagrams

(2 x 20 = 40)

29 (a) Trace the chronological development of the fermentation industry.

(OR)

(b) Describe in general the preparation of media for industrial fermentation.

30 (a) Discuss the application of computers in fermentation technology.

(OR)

(b) Bring out the application of chromatography in fermented product recovery and

purification.

 

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Loyola College B.Sc. Plant Biology and Biotechnology April 2009 Fermentation Technology Question Paper PDF Download

         LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.

VE 22

SIXTH SEMESTER – April 2009

PB 6606 / 6601 – FERMENTATION TECHNOLOGY

 

 

 

Date & Time: 23/04/2009 / 9:00 – 12:00   Dept. No.                                                   Max. : 100 Marks

 

                                   PART –A                                                    (20 marks)

 

  1. Choose the correct answer (5×1=5)

 

  1. The industry which uses the principle of fermentation is
  1. Food industry b. Paint industry c. Chemical industry   d. None

 

  1. The microbes are preserved in small laboratories by
  1. Daily Subculture b. Refrigeration c. Boiling        d. None

 

  1. The antifoam agent used  in fermentor is
  1. Soap b. Salt c. Lard oil    d. Vanaspati

 

  1. In the fermenter aeration is done by
  1. Sparger b. Agitators c. Impellors             d.  All

 

  1. The salt used in precipitation of enzymes is
  1. Ammonium sulphate b. Calcium sulphate c. Nickel sulphate   d. None

 

  1. State whether the following statements are True or False (5×1=5)

 

  1. Alcohol industry is the most profitable industry.

 

  1. Parasexual cycle in yeast is useful for strain improvement.

 

  1. Steam is the best sterilant for sterilisation of a fermenter.

 

  1. Sonication cannot be used for cell disruption.

 

  1. Biosensors are a boon to Fermentation industry.

 

III. Complete the following                                                                      (5×1=5)

 

  1. ______________ is a fermented dairy product.

 

  1. The sterilisation of heat sensitive substances is done by _________________.

 

  1. Cryopreservation is carried out at ____________°C temperature.

 

  1. The change in pH in media in the Fermentor is indicated by __________.

 

  1. The mixing of components of media in a fermentor is done by the _________ .

 

 

 

 

IV Answer the following within 50 words                                       (5×1=5)

Write short notes on

 

  1. Solvent extraction

 

  1.  DO probe

 

  1. Valves of fermentor

 

  1. Fed batch cultures

 

  1. Biotransformation

 

PART – B

Answer any FIVE of the following in about 350 words                       (5×8=40)

 

  1. Write on range of Fermentation processes available for industry.
  2. Explain Sterilization and its importance in fermentation.
  3. Describe the body construction and basic functions of each part of fermentor.
  4. Describe computerized control system available in fermentor
  5. Explain the use of chromatography in down stream processing
  6. Elaborate on the importance of effluent treatment after fermentation.
  7. Describe the scope and importance of recombinant products in fermentation.
  8. Precipitation is an important step in purification- Justify

 

PART-C

Answer the following in about 1500 words                                          (2X20=40)

 

  1. a. Write in detail about the different types of fermentors.

OR

  1. Describe the methods used in preservation of isolated cultures.

 

  1. a. Explain the filtration and centrifugation in Bioprocess industry.

OR

  1. Discuss in detail the Microbial growth kinetics and its use in fermentation .

 

 

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Loyola College B.Sc. Plant Biology and Biotechnology April 2011 Fermentation Technology Question Paper PDF Download

LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.

SIXTH SEMESTER – APRIL 2011

PB 6609/PB 6606 – FERMENTATION TECHNOLOGY

 

 

 

Date : 09-04-2011              Dept. No.                                        Max. : 100 Marks

Time : 9:00 – 12:00

 PART – A( 20 marks)

  ANSWER ALL THE QUESTIONS                                                                                                 

I.Choose the correct answer                                                                                                       (5×1=5)

  1. Amylases are produced by
  2. a) Aspergillus niger b) Bacillus licheniformis     c) Rhizopus       d) Phanerochaete

2.Air is supplied to a fermenter by

  1. a) Sparger b) Baffles               c) Impeller                      d) Shaft
  2. The preservation of cultures using freeze drying is called as
  3. a) Sterilisation b) Ionisation          c) Lyophilisation          d) Tyndallisation
  4. Antifoam agents used in industrial fermentations are
  5. a) Silicones b) Esters           c) Alcohols                                 d) All of these
  6. Cell disruption technique involves
  7. a) Centrifugation b) Precipitation           c) Filtration                    d) Liquid shear

 

  1. State whether the following statements are True or False           (5×1=5)
  2. Secondary metabolites are produced during idiophase.
  3. Batch culture is a closed culture containing limited amount of nutrients and inoculum.
  4. Impellers prevent swirling and vortexing of the fluid.
  5. Precipitationprocess is normally adopted for proteins.
  6. Tachometers are sensors which penetrate into the interior of the fermenter.

III. Complete the following                                                                                                         (5×1=5)

  1. An example of a product produced by a genetically engineered organism is __________.
  2. Air bubbles are distributed uniformly in the fermenter by___________ .
  3. The instrument used for measuring gas flow is ___________.
  4. The cheapest source of carbon available from plant source is_____________.
  5. The surfactant used in foam separation is________.

 

  1. Answer the following each in 50 words           (5×1=5)
  2. What are microbial metabolites?
  3. What are the components in a synthetic media?
  4. How will you adjust pH in a media?
  5. How will you control foam?

20.Comment on filtration in downstream processing.

 

                                                                 PART-B                                                             

Answer any FIVE of the following questions in about 350 words.                 (5×7=35marks)     

 Draw necessary Diagrams.

  1. a) Trace the development of industrial Biotechnology.

(OR)

  1. b) Explain microbial
  2. a) Write notes on the sterilization of media and equipment.

(OR)

  1. b) Discuss microbial growth kinetics in a batch culture.
  2. a) Describe the design of Airlift fermenter.

(OR)

  1. b) Describe the aeration and agitation devices attached to a fermenter.
  2. a) Discuss the methods of measuring dissolved oxygen.

(OR)

  1. b) Write notes on online control of fermenters.
  2. a) Comment on effluent treatment of industrial waste.

(OR)

  1. b) Explain the technique of Centrifugation in downstream processing.

 

PART – C

Answer any THREE of the following not exceeding 1200 words.                  (3×15=45 marks)

Draw diagrams wherever necessary.

 

  1. Explain the production and applications of microbial enzymes.
  2. Explain in detail the operation of a continuous culture fermenter and how does it differ from

a Batch fermenter.

  1. Describe the methods of preservation of industrially important microorganisms.
  2. Discuss the role of Biosensors in the fermentation industry.
  3. Describe the important processes in downstream processing.

 

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Loyola College B.Sc. Plant Biology and Biotechnology April 2012 Fermentation Technology Question Paper PDF Download

LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.

SIXTH SEMESTER – APRIL 2012

PB 6609/PB 6606 – FERMENTATION TECHNOLOGY

 

 

 

Date : 20-04-2012              Dept. No.                                        Max. : 100 Marks

Time : 1:00 – 4:00

 

PART – A

 

ANSWER THE FOLLOWING, EACH WITHIN 50 WORDS ONLY:                 (10 X 2=20 marks)

 

  1. Define fermentation.
  2.  What are secondary metabolites?
  3. What are the four phases of growth in fermentor?
  4. Define upstream processing.
  5. What are the functions of impeller in fermentor?
  6. Define sparger.
  7. What are steam traps?
  8. What is an online sensor?
  9. Comment on Trophophase and Idiophase.
  10. Write the principle of centrifugation.

 

PART – B

 

ANSWER THE FOLLOWING, EACH WITHIN 500 WORDS ONLY:

DRAW DIAGRAM WHEREVER NECESSARY:                                          (5 x 7 = 35 marks)

 

  1.  a) Write a general account on fermentation industries.

(or)

  1. b) Write short notes on transformation process.

 

  1.  a) What is fed batch fermentation?  Write explanatory notes on it.

(or)

  1. Describe the microbial growth kinetics in Continuous culture.

 

  1.  a) Write the Basic functions of a fermentor.

(or)

  1. b) Give an account on any two types of fermentor.

 

  1. a) Write about the measuring and controlling devices for monitoring of any four process

Variables.

(or)

  1. b) Write in short about the application of computers in fermentation.

 

 

 

 

  1. a) Give a detailed account on liquid-liquid extraction.

(or)

  1.  b) Schematically represent the process of down-stream processing.

 

 

PART – C

 

ANSWER ANY THREE OF THE FOLLOWING, EACH WITH 1200 WORDS:

DRAW DIAGRAM WHEREVER NECESSARY:                                                    (3X15=45 marks)

 

 

  1. Write an essay on range and component parts of a fermentation process.

 

  1.  Write a short note on (i) Isolation and Preservation of microorganism

 

(ii) Methods of improvement of industrial microbes.

 

  1. List out the essential parts of fermentor and write short note on each part.

 

  1. Write about the measuring and controlling devices for monitoring  pH, dissolved oxygen and foaming rate of a fermentor.

 

  1.  Write an essay on biosensors with their applications.

 

 

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