Loyola College B.Sc. Plant Biology and Biotechnology Nov 2006 Plant Biotechnology Question Paper PDF Download

            LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & BIOTECHNOLOGY

AE 12

FIFTH SEMESTER – NOV 2006

         PB 5504 – PLANT BIOTECHNOLOGY

(Also equivalent to PBB 514)

 

 

Date & Time : 30-10-2006/9.00-12.00         Dept. No.                                                       Max. : 100 Marks

 

 

 

 

Part A                                           (20 marks)

Answer All questions

I Choose the Correct Answer                                                                     ( 5 x 1 = 5 marks)

 

01.The reversion of mature state of plant cells into meristamatic cells (callus) under

     in vitro condition is called …..

  1. Differentiation b. Dedifferentiation    c. Redifferentiation     d. Totipotency

 

  1. Which of the following disinfectant is used in explant sterilization?
  2. Mercuric chloride b. Sodium hydroxide
  3. Tween d. Ammonia
  4. pBR 322 is constructed from ………..
  5. Yeast             b. E.coli           c. Rhizobium               d. Bacteriophage
  6. The gene for nodulin protein is encoded in ………
  7. nod gene b. nif gene       c. plant genome           d. both a and c.
  8. ……….. is used as stabilizers in ice cream.
  9. Sodium alginate b. Carageneen                         c. Agar            d. Sodium nitrate

 

II State whether the following statements are True or False                (5 x 1 = 5 marks)

  1. Autoclave is used to sterilize explants.
  2. Embryo culture technique is used to break the seed dormancy.
  3. Opines act as carbon and nitrogen source for the plants.
  4. CaMV has double stranded DNA.
  5. Cyanobacterium is commonly known as aquatic fern.

 

III. Complete the following                                                                     ( 5 x 1 = 5 marks)

  1. ………… is used to convert haploid into diploid plant.
  2. …….. is used to identify the regeneration of cell wall under in vitro condition.
  3. The phenolic compound synthesized by Agrobacterium infected plant is ……
  4. E. CoR1 cut DNA at ………. end.
  5. Spirulina contains ……… % of protein.

 

 

 

 

 

  1. Answer all, each in about 50 words ( 5 x 1 = 5 marks)
  2. Define somaclones.
  3. What do you mean by encapsulation of embryo?
  4. Distinguish between Ti plasmid and Ri plasmid.
  5. Define Genomic library.
  6. Mention the alga, which is used in cosmetics.

PART B                       (5 x 8 = 40 marks)

Answer any five, each within 350 words only. Draw diagrams and flowcharts wherever necessary.

  1. Explain the basic steps in plant tissue culture.
  2. Discuss in brief the biotechnological applications of plant cell and tissue culture in

plant sciences.

  1. Expand the following with a footnote
  2. DMSO b. PEG                        c. SDS-PAGE             d. HEPA
  3. pUC f. IBA             g. TEMED                  h. BAP
  4. Discuss the potentials of Ti plasmid of Agrobacterium tumefaciens in the genetic

engineering of plants.

  1. With the suitable diagram, describe electrophoresis.
  2. Describe the physical methods of gene transformation in plants.
  3. Define phycocolloids. Enumerate the significance of phycocolloids.
  4. What is phycoculture? Describe any two methods of phycoculture.

PART C                       ( 2 x 20 = 40 marks)

Answer the following, each within 1500 words only. Draw diagrams and flowcharts wherever necessary.

 

  1. a). i) What are somaclonal variations? (5 marks)
  2. ii) Write down the steps involved in cryopreservation.(7 marks)

iii) How do you produce artificial seeds under in vitro condition? (8 marks)

Or

b). i) Define totipotency. Explain the concept of totipotency and its exploitation in

Biotechnology.( 12 marks)

  1. ii) Discuss the role of plant growth regulators in plant tissue culture. (8 marks)
  2. “Chloroplast and Mitochondria are called as semiautonomous organelles” – Justify.

Or

Define Genetic Engineering. Elaborate on genetic engineering for insect resistance.

 

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Loyola College B.Sc. Plant Biology and Biotechnology April 2007 Plant Biotechnology Question Paper PDF Download

    LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.

IB 31

FIFTH SEMESTER – APRIL 2007

PB 5504 – PLANT BIOTECHNOLOGY

 

 

 

Date & Time: 30/04/2007 / 1:00 – 4:00         Dept. No.                                                       Max. : 100 Marks

 

 

Part A                                           (20 marks)

Answer All questions

I Choose the Correct Answer                                                                     ( 5 x 1 = 5 marks)

 

  1. Which one of the following media is most commonly used in plant tissue culture?
  2. Whites medium b. B5 medium    c. Nitsch medium     d. M.S. medium

 

  1. What term is given to the ability of a cell regenerated into a plantlet?
  2. callus b. totipotency              c. meristem                  d. explant

 

  1. A small fragment of radio-labeled DNA molecule complementary to the foreign

DNA is called…

  1. cDNA b. DNA probe             c. palindromic DNA   d. T- DNA

 

  1. Function of nod gene is ….
  2. nitrogen fixation b. nodule development
  3. nitrogen assimilation d. nitrogenase production

 

  1. The purified form of agar is ……
  2. cellulose b. agarose                    c. alginate                    d. sucrose

 

II State whether the following statements are True or False                (5 x 1 = 5 marks)

 

  1. Laminar air flow cabinet consists of HEPA filter.
  2. Knudson medium is used for orchid culture.
  3. The opine synthesized by Nop. Ti plasmid is acetosyringone.
  4. pBR 322 is constructed from pUC.
  5. Sodium alginate is used as fusogent.

 

III.  Complete the following                                                                    ( 5 x 1 = 5 marks)

  1. Hot air oven is used for …………. of glassware.
  2. PEG refers to …………
  3. Agrobacterium tumefasciens causes a disease called ………
  4. The function of APS in Electrophoresis is ………
  5. Spirulina contains ……… pigment.

 

 

 

 

  1. Answer all, each in about 50 words ( 5 x 1 = 5 marks)

 

  1. Define Micropropagation.
  2. What is explant?
  3. Distinguish between Ti and Ri plasmid.
  4. Define molecular probe.
  5. What is single cell protein?

PART B                       ( 5 x 8 = 40 marks)

 

Answer any five, each within 350 words only. Draw diagrams and flowcharts wherever necessary.

  1. Briefly explain the various sterilization techniques in plant tissue culture.
  2. What is acclimatization? Explain how in vitro regenerated plants are transferred to

natural condition.

  1. Define callus. Explain the types of callus. Add a note on its significance.
  2. Define: a. Organogenesis b. Encapsulation of embryo
  3. Cybrid d. Dedifferentiation.
  4. Explain briefly the molecular interaction between Rhizobium and legume plants.
  5. What is Taq DNA polymerase? Write down the steps involved in PCR.
  6. Describe any two methods of direct gene transformation in plants.
  7. Write notes on phycocolloids.

 

PART C                       ( 2 x 20 = 40 marks)

 

Answer the following, each within 1500  words only. Draw diagrams and flowcharts wherever necessary.

 

29a. Mention the significance of plant tissue culture techniques in plant sciences.

 

Or

  1. Give an account of somatic hybridization. Add a note on its significance of

cybrids.

 

30a. i. Describe the structure and function of mitochondrial genome.

  1. Explain the method of southern blotting.

Or

  1. i. Explain how Ti plasmid is exploited in plant biotechnology.
  2. Explain the role of yeast in gene cloning.

 

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Loyola College B.Sc. Plant Biology and Biotechnology April 2009 Plant Biotechnology Question Paper PDF Download

      LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.

VE 18

FIFTH SEMESTER – April 2009

PB 5504 – PLANT BIOTECHNOLOGY

 

 

 

Date & Time: 24/04/2009 / 1:00 – 4:00  Dept. No.                                                      Max. : 100 Marks

 

 

PART – A      (20 marks)

  1. Choose the correct answer: (5 x 1 = 5)

 

  1. In plant tissue culture commonly used medium is
  2. S. medium b) Nitsch medium       c) B5 medium             d) white’s medium
  3. Laminar airflow cabinet consists of
  4. HAPA filter b) HEPA filter            c) HEAP filter d) HAPE filter
  5. Most suitable explant for the production of haploid plant is
  6. ovule b) anther          c) microspore   d) stamen
  7. Cyanobacteria are commonly called as
  8. Red algae b) Green algae c) Blue algae   d) Blue green algae
  9. The purified form of agar is
  10. agarose b) Sucrose       c) cellulose      d) alginate

 

  1. State the following statements are true or false: (5 x 1 = 5)

 

  1. T-DNA refers to transfer DNA.
  2. Sodium alginate is used as fusogent.
  3. Phenosafranin is used to identigy dead protoplast.
  4. pBR 322 is constructed from PUC.
  5. Agar-agar is extracted from the algae Gracilaria.

 

  • Complete the following: (5 x 1 = 5)

 

  1. Taq DNA polymerase enzyme is extracted from __________________.
  2. sodium alginate is extracted from _________________.
  3. Spirulina contains ________________ pigment.
  4. Knudson medium is used for _______________ culture.
  5. Single stranded DNA is present in ______________ virus.

 

  1. Answer all in about 50 words: (5 x 1 = 5)

 

  1. What is an explant?
  2. Define micropropagation.
  3. What is single cell protein?
  4. Mention any two algal seaweeds.
  5. What is chimeric DNA?

 

 

PART – B

 

Answer any FIVE, each within 350 words. Draw diagrams/ flow charts wherever necessary:  

                                                                                                                                       (5 x 8 = 40)

 

  1. Describe the Polymerase chain Reaction technology.
  2. Enumerate the significance of Blue Green Algae.
  3. Write notes on: a) artificial seed                       b)restriction enzyme
  4. Briefly explain the various physical method of sterilization techniques.
  5. Describe the genetic organization and function of chloroplast genome.
  6. Give the biotechnological importance of yeast.
  7. Write notes on phycocolloids.
  8. Define callus. Describe the types and add a note on its significance.

 

PART – C

 

Answer the following each within 1500 words only. Draw diagrams/ flow charts wherever necessary:                                                                                                                      (2 x 20 = 40)

 

 

  1. a) Mention the significance of tissue culture in plant science

(OR)

  1. b) Explain the genetic aspects involved in nitrogen fixation in Legume-Rhizobium

interaction.

  1. a) Give an account of somatic hybridization. Add a note on its significance.

(OR)

  1. b) What is rDNA technology? Describe any four gene transfer techniques used in rDNA

technology.

 

 

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Loyola College B.Sc. Plant Biology and Biotechnology Nov 2010 Plant Biotechnology Question Paper PDF Download

LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

   B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.

FIFTH SEMESTER – NOVEMBER 2010

PB 5518/PB 5512/PB 5504 – PLANT BIOTECHNOLOGY

 

 

 

Date : 09-11-10                     Dept. No.                                                   Max. : 100 Marks

Time : 9:00 – 12:00

PART – A (20 Marks)

 

  1. Choose and circle the best answers of the following:                                      (5 x 1= 5)

 

  1. Biotechnology can be defined as

(a) Science and technology                       (b) Biology and technology

(c) Production of a product from biological organisms using technology

(d) Production of a product using different technology from plants and animals

 

  1. Early attempts to derive callus, scientist used

(a) Tobacco pith cells and carrot cambial cells               (b) Cauliflower

(c) Shoot tip of tobacco                                                       (d) Carrot cambial cells

 

  1. Virus free plants can be derived using
  • Embryo culture (b) Mericulture          (c) Anther Culture    (d) Leaf disc culture

 

  1. The most conventional and natural method for gene transfer is by

(a) Biolistic gun  (b) Microinjection    (c) Agrbacterium mediated     (d) Electroporation

 

  1. GUS gene used in DNA technology is

(a) Marker gene        (b) Molecular marker    (c) Silence gene     (d) Jumping gene

 

  1. State whether the statements are TRUE or FALSE.                         (5 x 1 = 5)

 

  1. Sodium Hypochloride is a surface sterilising agent.
  2. Immobilised cells are called as synthetic seeds.
  3. Anabaena is a soil bacteria used as vector in Bt cotton.
  4. Taq DNA polymerase cleaves deoxyribonucleic acid.
  5. QTL is one the target for crop improvement.

   III. Complete the following                                                                                            (5 x 1 = 5)     

 

  1. Determination of cell growth by turbidity in a suspension culture system is known as

_________.

 

  1. A ring like vascular structure that develops inside callus is called as __________.

 

  1. Agrabacterium tumifaciens when causes Crown gall disease in plant the T-DNA triggers

the production of ____________ and ___________.

 

  1. Most important enzyme that is used in the construction of cDNA library is _______.

 

  1. Most important molecular tool in Crop Improvement studies is _____________.

 

  1. Answer the following, each within 50 words only.             (5 x 1 = 5)

 

  1. What is an “explants”?
  2. Define Caulogenesis
  3. What is nif gene?
  4. Explain gene library
  5. Expand GMO? Give an example.

 

PART – B

 

Answer the following, each answer within 500 words. Draw diagrams and flowcharts

     wherever necessary.                                                                                             (5 x 7 =35 Marks)

 

  1. a) Write the historical mile stone of tissue culture in brief.

(OR)

  1.        b) Write short notes on components of tissue culture media.

 

  1. a) Describe how synthetic seeds are produced.

(OR)

  1.       b) Give short account on Anther culture.

 

  1. a) How Rhizobium interacts with legume plants at the molecular level.

(OR)

  1.       b) Write short notes on organization and function of Plant nuclear genome

 

  1. a) Define restriction enzyme. Explain its use in recombinant DNA technology

(OR)

  1.       b) Explain the process of Polymerase Chain Reaction in gene amplification

 

  1. a) What are various biotechnological strategies applied for crop improvement.

(OR)

  1.       b) Write the role of molecular markers in crop improvement.

 

              PART – C

 

Answer any three of the following, each with 1200 words. Draw diagrams and flowcharts

  wherever necessary.                                                                                                (3 x 15 = 45 Marks)

 

  1. Give a descriptive account on callus and cell suspension culture techniques.
  2. How protoplast is isolated, fused/manipulated and cultured.
  3. Describe how Agrobaterium is used in gene transfer.
  4. What are the different methods by which genes are directly transferred in to plants?

Describe methodology involved in Particle gun bombardment method.

 

  1. Write a detailed protocol to produce insect and herbicide resistant transgenic plants.

 

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Loyola College B.Sc. Plant Biology and Biotechnology April 2011 Plant Biotechnology Question Paper PDF Download

LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.

FIFTH SEMESTER – APRIL 2011

PB 5518/PB 5512/PB 5504 – PLANT BIOTECHNOLOGY

 

 

 

Date : 20-04-2011              Dept. No.                                                  Max. : 100 Marks

Time : 1:00 – 4:00

 

PART-A (20 Marks)

Answer ALL  questions

 

  1. Choose the correct answer                                                                                        (5X1=5 marks)

 

  1. ______ is the ability of a single cell to divide and produce all the differentiated cells in an organism, including extraembryonic tissues.
  2. Micropropagation   Totipotency            c. Pluripotency.         d. clonal propagation

 

  1. Cultures are generally initiated from sterile pieces of a whole plant termed __________
  2. Implant   explant                    c. protoplast                d. None

 

  1. The molecular size of Ti plasmid is ______
  2. 20 Kb                   100 Kb                    c. 200 Kb                     d. 2 Kb

 

  1. The principal component in chain termination method of DNA sequencing is _______
  2. Deoxynucleotides            Ribonucleotides        c. Dideoxynucleotides          d. All

 

  1. The nptII antibiotic marker used in plants impart resistance to ________
  2. Kanamycin                       Ampicillin                c. Chloramphenicol                d. Penicillin

 

  1. State whether the following statements are TRUE or FALSE (5X1=5 marks)

 

  1. GA3 is a form of Gibberellic acid.
  2. Callus culture is often performed in the dark.
  3. Opines are condensation products of fatty acids and sugars.
  4. Reverse transcriptase is RNA dependant DNA polymerase.
  5. RAPD is Restriction amplified Polymorphic DNA.

 

  • Complete the following (5X1=5 marks)

 

  1. _______ is defined as the mass of undifferentiated tissue.
  2. _________is a technique by which protoplasts of plant cells belonging to different species or genera are fused.
  3. During nodule formation, Rhizobia in the host cell cytoplasm,   get transformed into swollen, irregular-shaped, branched structures called  ______
  4. The transfer of DNA from gel to membrane is called _______
  5. The genome of _______ plant was the first candidate for complete genome sequencing.

 

  1. Define the following (5X1=5 marks)

          

  1. Define Acclimatization.
  2. What are somaclonal variants?
  3. RUBISCO-Explain.
  4. Define Gene library.
  5. Abiotic stress.

PART-B

 

Answer the following  within 500 words. Draw  diagrams wherever necessary                  (5X7=35 marks)

 

 

  1. a) Discuss the role of hormones in in vitro culture.

 

(or)

 

  1. b) Write short notes on the different types of media used in plant tissue culture.

 

  1. a) Briefly explain the steps involved in the isolation of protoplasts.

 

(or)

 

  1. b) Explain in short about the anther culture.

 

  1. a) Explain the organization and function of chloroplast DNA.

 

(or)

 

  1. b) Write notes on : i) CMS              ii) Rhizobial genes.                                          (4 + 3 marks)

 

  1. a) Write notes on:
  2. Restriction enzymes ii. Molecular probes.                           (4+3 marks)

 

(or)

 

  1. b) Explain briefly about the gene delivery systems.

 

  1. a) Elaborate on the production of insect resistant transgenic plants.

 

(or)

 

  1. b) Production of herbicide resistant transgenic plants- Explain.

 

PART-C

 

Answer any THREE of the following, each with 1200 words.  Draw diagrams wherever necessary

(3X15=45 marks)

  1. Elaborate on the sterilization methods employed in plant tissue culture.
  2. Describe in detail about somatic embryogenesis & synthetic seeds. Also add a note on their

applications.

  1. Explain the molecular mechanism involved in the transformation of plants by Agrobacterium

      tumefaciens.

  1. Explain in detail about the principle and applications of Polymerase chain reaction.
  2. Give a detailed account on the molecular markers and DNA fingerprinting.

 

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Loyola College B.Sc. Plant Biology and Biotechnology April 2012 Plant Biotechnology Question Paper PDF Download

LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.

FIFTH SEMESTER – APRIL 2012

PB 5518/PB 5512/PB 5504 – PLANT BIOTECHNOLOGY

 

 

 

Date : 03-05-2012              Dept. No.                                        Max. : 100 Marks

Time : 9:00 – 12:00

 

                                                                   PART –A                                  

 

Answer ALL questions;  Each within 50 words only:                                    (10×2=20marks)

 

  1. Define biotechnology.
  2. What is Aseptic condition?
  3. Define Caulogenesis.
  4. What is a Fusogen?
  5. What are nif genes?
  6. What is T-DNA?
  7. What are cloning vectors?
  8. What are Microprojectiles?
  9. Give the use of npt II gene.
  10. What are cry genes?

     PART –B                                  

 

Answer the following each in 500 words only;

Draw diagrams and flow charts wherever necessary:                                    (5×7=35 marks)

 

  1. a) Give a brief account on sterilization methods.

(OR)

  1. b) Write short note on media composition.

 

  1. a) Describe the procedure used for embryo culture.

(OR)

  1. b) Give an account on somatic embryogenesis.

 

  1. a) Explain the genetic organisation of mitochondrial DNA.

(OR)

  1. b) Give a brief note on cytoplasmic male sterility.

 

  1. a) Describe various cloning vectors.

(OR)

  1. b)  Write short note on any one blotting technique.

 

  1. a) Give the strategies used for the development of transgenic plants against herbicides.

(OR)

  1. b) Write briefly on genome mapping.

 

 

 

 

   PART –C                         

 

Answer ANY THREE of the following, each within 1200 words only;       (3 x 15 = 45 marks)

Draw diagrams and flow charts wherever necessary:

 

  1. Explain in detail on cell and suspension culture. Add a note on its applications.
  2. Write a detailed account on meristem culture. Add a note on its importance in the field

of agriculture.

  1. Write an essay on Agrobacterium mediated gene transformation.
  2. Describe in detail on various direct gene delivery methods.
  3. Give a detailed account on molecular markers.

 

 

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Loyola College B.Sc. Plant Biology and Biotechnology Nov 2012 Plant Biotechnology Question Paper PDF Download

LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.

FIFTH SEMESTER – NOVEMBER 2012

PB 5518/5512/5504 – PLANT BIOTECHNOLOGY

 

 

 

Date : 08/11/2012             Dept. No.                                        Max. : 100 Marks

Time : 9:00 – 12:00

 

PART – A

 

ANSWER THE FOLLOWING, EACH WITHIN 50 WORDS:                           (10 x 2 = 20 marks)            

  1. Define callus.
  2. What is an explant?
  3. What is a cybrid?
  4. Define vector.
  5. What are nod genes?
  6. Distinguish between intron and exons.
  7. Mention the role of Restriction endonuclease Type II.
  8. Define microinjection.
  9. Write notes on RAPD.
  10. What is Bt-gene?

 

PART – B     

 

ANSWER THE FOLLOWING, EACH WITHIN 500 WORDS.

DRAW DIAGRAMS, FLOWCHARTS WHEREVER NECESSARY:              (5 x 7 = 35 marks)

 

 

  1. a) Enumerate the importance of Plant tissue culture.

(OR)

  1. b) Give a brief account on the methods of sterilization.

 

  1. a) How are artificial seeds produced under in vitro condition?.

(OR)

  1. b) Write notes on cryopreservation.

 

  1. a) Describe the structure and organization of chloroplast DNA.

(OR)

  1. b) Write an account on the genetic organization of Ti plasmids.

 

  1. a) Briefly explain Polymerase Chain Reaction.

(OR)

  1.  b) Write short notes on

(i)   Cloning vectors

(ii)  Genomic library

 

  1. a) Give an account on DNA finger printing.

(OR)

  1. b) Write notes on importance of molecular markers in genome mapping.

 

PART – C     

 

ANSWER ANY THREE OF THE FOLLOWING, EACH WITHIN 1200 WORDS.

DRAW DIAGRAMS, FLOW CHARTS WHEREVER NECESSARY:               (3 x 15 = 45 marks)

 

  1. Write an account on callus culture and mention its significance.
  2. Give an illustrated account of Isolation and fusion of protoplasts.
  3. Describe the molecular interaction of Rhizobium with leguminous plants.
  4. Write an essay on Agrobacterium mediated gene transfer.
  5. Describe the development of transgenic plants with reference to insect resistance.

 

 

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