LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & BIOTECHNOLOGY
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FIFTH SEMESTER – NOV 2006
PB 5400 – MICROBIAL TECHNOLOGY
(Also equivalent to PBB 400)
Date & Time : 03-11-2006/9.00-12.00 Dept. No. Max. : 100 Marks
Part A
Answer all the questions (20 marks)
- Choose the correct answer (5 x 1 = 5 )
- _____ is used both as a carbon source and nitrogen source
- a) Sucrose b) Molassus
- c) Whey d) Soybean meal
- Beer production does not involve
- a) carbonation b) distillation c) filtration d) clarification
- Auxotrophic mutants of yeast are used in the production of
- a) Hepatitis B vaccine b) Erythopoietin c) Interferon α d) Insulin
- Which of the following is used in the production of acetic acid?
- a) Glucanobacter b) Aspergillus c) Bacillus d) Penicillium
- Azotobacter is used in the production of
- a) biopesticide b) biofertilizer c) biosorban d) biopolymer
- State whether the following are True or False (5 x 1 = 5 )
- Biosensors are used for online monitoring.
- The protein content of Spirulina is 80%.
- Streptomycin is a peptide containing antibiotic.
- Xylanase can be used in bleaching of paper.
- Mycorrhizae help to increase the uptake of phosphorus.
III. Complete the following (5 x 1 = 5)
- Turbulance in a fermentor is controlled using ________.
- Hops is the female cone of _________ _________.
- CNBr is used to cleave the peptide at ________ residues.
- Glucanobacter is used for the production of __________________.
- Dextran is produced using ______.
- Answer the following in about 50 words (5 x 1 = 5)
- Define cellular yield coefficient.
- What is yogurt?
- Mention any four microbes used in production of Riboflavin.
- What is aspartame?
- Define gasohols.
Part B
Answer any five of the following each in about 350 words. Draw necessary diagrams.
(5 x 8 = 40)
- Give an account of the range of products obtained from microbe-based industries.
- Write notes on the carbon sources used in formulating media.
- Elaborate the steps involved in beer production.
- Briefly discuss about the production of Spirulina and its importance.
- Give an account of bioconversion.
- List out the various industrially important enzymes, their sources and applications.
- Explain the steps involved in production of citric acid.
- What are biopolymers? Explain the source and uses of any four
Part C
Answer the following questions in about 1500 words. Draw necessary diagrams
(2 x 20 = 40)
- a) Elucidate the structure and functioning of a typical fermentor. List out the salient features of an
ideal fermentor.
(or)
- Give a detailed account of cheese production. Add a note on their types.
- a) Explain: a) Production of Penicillin b) Production of Hepatitis B vaccine.
(or)
- What are biofertilizers? Give a detailed account of the types of biofertilizers and their applications.
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