LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & BIOTECHNOLOGY

AE 08

FIFTH SEMESTER – NOV 2006

PB 5403 – PLANT BIO-CHEMISTRY

 

 

Date & Time : 06-11-2006/9.00-12.00  Dept. No.                                                       Max. : 100 Marks

 

 

 

Part A                                           (20 marks)

Answer All questions

 

I Choose the Correct Answer                                                                     ( 5 x 1 = 5 marks)

 

1. Solution having pH 6 is …….
2. acidic b. highly acidic            c. alkaline                    d. highly alkaline.
3. Which of the following one belongs to ketone group.
4. Dihydroxyacetone b. Glucose       c. Pentose        d. Fructose
5. Mention the amino acid having symmetric α – carbon.
6. Glycine b. Serine          c. Methionine              d. Cystine
7. Pick the odd one out.
8. Fats b. Waxes         c. Glycolipids              d. Terpenoids
9. Nomenclature of an enzyme is assigned with …… numbers.
10. 1                       b. 2                  c. 3                  d. 4

 

 

II State whether the following statements are  True or False    ( 5 x 1 = 5 marks)

6. The pH of the blood higher than the normal condition is called alkalosis.
7. Polysaccharides are sweet in taste.
8. Globular proteins are soluble in water.
9. Saturated fatty acids do not contain double bond.
10. Enzyme with organic molecule is called coenzyme.

III. Complete the following                                                                     ( 5 x 1 = 5 marks)

11. The working principle of a colorimeter is based on …. and ……… law.
12. Covalent linkage between monomers of a polysaccharide is called ……….
13. The R group in Glycine is …..
14. Competitive inhibitor for succinic acid is …..
15. Fatty acids with one double bond are known as …………

 

1. Answer all, each in about 50 words ( 5 x 1 = 5 marks)
2. Expand SDS – PAGE.
3. What is inulin?
4. Mention any two aromatic amino acids.
5. What is lecithin?
   20. Define V- max.

 

 

PART B                       ( 5 x 8 = 40 marks)

 

Answer any five, each within 350 words only. Draw diagrams and flowcharts wherever necessary.

 

21. Define biomolecule. Describe the structure of an atom.
22. What is a bond? Explain ionic, covalent, coordinate and glycosidic bond.
23. Describe the classification of amino acids.
24. Write notes on sucrose and maltose in plants.
25. Give an account of classification of proteins.
26. Define holoenzymes. Explain the various factors affecting the enzyme activity.
27. Explain the types of secondary metabolites. Add a note on their significances.
28. What is Michalies – Menton constant? Explain the enzyme kinetics and Km value .

 

PART C                       ( 2 x 20 = 40 marks)

 

Answer the following, each within 1500 words only. Draw diagrams and flowcharts wherever necessary.

 

29. a) Explain the working principle and design of any four-biochemical techniques.

 

Or

 

1. b) Write an essay on general classification, structure and properties of carbohydrates.

 

30. a) Describe the various structure of a protein. Enumerate the biological role of

peptides.

 

Or

 

1. b) Give an account of:
2.   classification of lipids and fatty acids. ( 12 marks)
3. oxidation of fatty acids.. (8 marks)

 

 

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