LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

IB 27

B.Sc  DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.

FIFTH SEMESTER – APRIL 2007

PB 5403 – PLANT BIO-CHEMISTRY

 

 

Date & Time: 04/05/2007 / 1:00 – 4:00          Dept. No.                                                     Max. : 100 Marks

 

 

 

Part A                                           (20 marks)

Answer All questions

 

I Choose the Correct Answer                                                                     ( 5 x 1 = 5 marks)

1. The pH denotes the relative concentration of …… ions in a solution.
2. hydrogen b. hydroxyl                  c. oxygen         d. both a and b

 

2. Pick the odd one out.
3. Starch b. Cellulose                 c. Glycogen     d. Glucose

 

3. The number of peptide bond present in a tripeptide is ….
4. 1 b. 2                  c. 3                  d. 4

 

4. Name the additional group present in Phospholipids.
5. phosphoric acid b. sulphuric acid          c. lecithin         d. citric acid

 

5. Mention the site at which substrate binds with an enzyme.
6. active site b. N-terminal               c. Carboxyl end           d. both b and c

 

 

II State whether the following statements are  True or False    ( 5 x 1 = 5 marks)

6. TEMED acts as catalyst in SDS-PAGE.
7. Sucrose is the condensation of two molecules of fructose.
8. The R group in Glycine is hydrogen.
9. An alcohol group in lecithin is glycerol.
10. Enzyme involved in oxidation-reduction reaction is called oxidoreductase.

 

III. Complete the following                                                                     ( 5 x 1 = 5 marks)

11. ……….. force is involved in centrifugation.
12. The reserved carbohydrate in cell wall is …….
13. Tryptophan is a …………. amino acid.
14. Esters of fatty acids with other than glycerol is called as ……..
15. Km refers to ………..

 

1. Answer all, each in about 50 words ( 5 x 1 = 5 marks)

 

16. Expand TLC and GLC.
17. Define Disaccharide.
18. What is β – sheet structure?

 

19. Define glycolipid.

 

20. What is active site?

PART B                       ( 5 x 8 = 40 marks)

Answer any five, each within 350 words only. Draw diagrams and flowcharts wherever necessary.

 

21. Define biomolecule. Explain how chemical bonding occurs in biomolecules.
22. Explain the working principle of centrifuge. Add a note on various types of

centrifuge.

23. Describe the structure and properties of cellulose and starch.
24. Briefly explain the structure of protein.
25. Give an account of classification of amino acids based on R group.
26. Explain the mode of action of enzymes.
27. Write notes on saturated and unsaturated fatty acids.
28. Describe the classification of enzymes.

PART C                       ( 2 x 20 = 40 marks)

 

Answer the following, each within 1500  words only. Draw diagrams and flowcharts wherever necessary.

 

29a.  Give a detailed account of different supporting media used in  electrophoresis.

 

Or

1. Explain the structure and properties of monosaccharide and disaccharides.

 

30a. Give an account on the classification and properties of protein.

 

Or

 

1. i.  What is an enzyme? Describe the general classification and enzyme kinetics.
2. Explain the types of enzyme inhibitors.

 

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