LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.
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FIFTH SEMESTER – November 2008
PB 5408 – BIOPHYSICS
Date : 14-11-08 Dept. No. Max. : 100 Marks
Time : 9:00 – 12:00
PART A (20 marks)
Answer ALL questions
I Choose the Correct Answer (5 x 1 = 5 marks)
- Which among the following are important characteristics of gases?
- a) gases are highly compressible b) gases are thermally expandable
- c) most gases have low densities d) all the above
- Which among the following is acidic range for pH.
- a) 2-5 b) 7 8-12 d) none of the above
- Example of liquid-in-liquid colloids
- ice cream b. paint c. milk d. ink
- A 0.5M solution of NaCl (molecular weight-59) is prepared by adding___________
grams of NaCl in 1 litre of water.
- a) 59.00 b) 29.50 c) 118.00 d) 14.75
- __________is a branch of physics which deals with the energy and work of a system.
- a) radiation b) light c) isotopes d) Thermodynamics
II State whether the following statements are True or False (5 x 1 = 5 marks)
- Ultrafiltration used to improve the water quality.
| 07. Volume percent = | volume of solute volume of solution |
x 100. |
- Plasmids are positively charged molecules.
- 400-700nm is the wave length of the visible region of the electromagnetic radiation.
- Isotopes are same elements with different atomic mass.
III. Complete the following (5 x 1 = 5 marks)
- Diffusion refers to the process by which molecules intermingle as a result of their
____________ of random motion.
- Dialysis tube is made up of _______________.
- Immunoelectrophoresis is also called ______________ electrophoresis.
- Optical activity is measured using a _______________.
- __________occurs when the neutron to proton ratio is too great in the nucleus and
causes instability.
- Answer ALL, each in about 50 words (5 x 1 = 5 marks)
- What is Bernoulli’s equation?
- Define buffers.
- Explain absorbents.
- What is the function of Dichroic Mirror?
- What is radiosensitivity?
PART B (5 x 8 = 40 marks)
Answer any five, each within 350 words only. Draw diagrams and flowcharts wherever necessary.
- Write notes on phases and its types.
- Describe the following
- i) Normality
- ii) Mole fraction
iii) Determination of osmotic pressure.
- Define dialysis. How it works and its uses.
- Write the principle, mechanism and uses of Photometry.
- Elaborated notes on viscosity.
- Write notes on the following
- i) Eelctron Gun.
- ii) Sample preparation method for TEM.
- Write brief on Beer and Lambert’s law.
- Describe the three types of radioactive decay.
PART C (2 x 20 = 40 marks)
Answer the following, each within 1500 words only. Draw diagrams and flowcharts wherever necessary.
- a) Structure, Working principle and uses of SDS PAGE electrophoresis.
(OR)
- b) Write note on the following
- i) Write notes on two types of ultracentrifugation.
- ii) Immunoelectrophoresis and its types.
- a) Describe the following chromatography techniques and its uses
- i) Thin layer
- ii) Gel filteration
iii) Gas
(OR)
- b) Write detailed notes on the following
- i) Law of Thermodynamics.
- ii) Structure, function and uses of Fluorescence microscope.
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