Senior School Certificate Examination (XI-XII) Biotechnology 2018-19

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CBSE Curriculum for Senior School Certificate Examination (XI-XII) Biotechnology 2018-19

10. BIOTECHNOLOGY (Code No. 045) CBSE
An unprecedented growth of human knowledge in the field of Biological Sciences coupled with equally
significant developments in the field of technology have brought significant changes into existing social and economic systems. The emerging field of Biotechnology is likely to further enhance the applications of Science and Technology in the service of human welfare. Modern Biotechnology processes encompass a wide range of new products such as antibiotics, vaccines, monoclonal antibodies and many more. Furthermore, developments in recombinant DNA technology have yielded numerous new useful products in the fields of healthcare and agriculture. The present syllabus takes care of all these aspects. Due emphasis has been laid on familiarizing the learners with the fundamental concepts, basic techniques and their applications. It is expected that the knowledge gained through the study of different topics and the skills acquired through the prescribed practical work will make the learners competent to meet the challenges of academic as well as professional courses after studying the subject at senior secondary stage.
Objectives
The broad objectives of teaching Biotechnology at senior secondary level are:
To help the learners know and understand basic facts and concepts of the subject at elementary stage.
To expose the students to different basic processes and basic techniques used in Biotechnology. To familiarize the learners to understand the relationship of the subject to health, nutrition,
environment, agriculture and industry, etc.
To develop conceptual competence in the learners so as to cope up with professional courses in future career.
To acquaint students with different applications of Biotechnology in everyday life.
To develop an interest in students to study biotechnology as a discipline.
CBSE COURSE STRUCTURE
CLASS – XI (2018-19)
One Paper                                                                                                                                  Time: 3 hrs.
Max. Marks 70+30
Units No. of Periods Marks
Unit- I Biotechnology: An overview 20 5
Unit-II Molecules of Life 50 20
Unit-III Genetics and Molecular Biology 50 20
Unit-IV Cells and Organisms 60 25
Practicals 60 30
Total 240 100
CBSE CLASS XI
(Theory)
One Paper                                                                                                                                        Time: 3 hrs.
Total Marks :70                                         180 Periods
Unit-I Biotechnology: An overview                                                            5 Marks                  20 Periods
Chapter 1: Biotechnology: An Overview
Historical Perspectives, Technology and Applications of Biotechnology, Global market and Biotech Products, Public Perception of Biotechnology, Biotechnology in India and Global Trends
Unit-II Molecules of Life                                                                               20 Marks                 50 Periods
Chapter 1: Biomolecules: Building Blocks
Building Blocks of Carbohydrates – Sugars and Their Derivatives, Building Blocks of Proteins – Amino Acids, Building Blocks of Lipids – Simple Fatty Acids, Sphingosine,
Glycerol and Cholesterol, Building Blocks of Nucleic Acids – Nucleotides, Biochemical Transformations
Chapter 2: Macromolecules: Structure & Function
Carbohydrates – The Energy Givers, Proteins – The Performers, Enzymes – The Catalysts, Lipids and Biomembranes – The Barriers, Nucleic Acids – The Managers
Unit-III Genetics and Molecular Biology                                                  20 Marks                   50 Periods
Chapter 1: Concepts of Genetics
Historical Perspective, Multiple Alleles, Linkage and Crossing Over, Genetic Mapping, Gene Interaction,
Sex-Linked Inheritance, Extranuclear Inheritance, Quantitative Inheritance, Genes at the Population Level
Chapter 2: Genes and Genomes: Structure and Function Discovery of DNA as Genetic Material, DNA
Replication, Fine Structure of the Genes, From Gene to Protein, Transcription – The Basic Process,
Genetic Code, Translation, Regulation of Gene Expression, Mutations, DNA Repair, Human Genetic Disorders, Genome Organization
Unit IV: Cells and Organisms                                                                       25 Marks                  60 Periods
Chapter 1 The Basic Unit of Life
Cell Structure and Components, Tissues and Organs, Stem Cells, Biodiversity, Organization of Life
Chapter 2: Cell Growth and Development
Cell  Division,  Cell  Cycle,  Cell  Communication,  Nutrition,  Gaseous  Exchange,  Internal  Transport, Maintaining  the  Internal  Environment,  Reproduction,   In  Vitro  Fertilization,  Animal  and  Plant
Development, Immune Response in Animals, Programmed Cell Death, Defense Mechanisms in Plants
CBSE PRACTICALS                                                                                                                                         60 Periods
Note: Every student is required to do the following experiments during the academic session.
1.   Recording practical results and safety rules in the laboratory
2.   Preparation of buffers and pH determination
3.   Sterilization techniques
4.   Preparation of bacterial growth medium
5.   Determination of bacterial growth curve
6.   Cell counting
7.   Isolation of milk protein (Casein)
8.   Estimation of whey protein by biuret method
9.   Assaying the enzyme acid phosphate
10. Estimation of blood glucose by enzymatic and glucometer method (GOD/POD)
11. Study of various stages of mitosis and calculation of mitotic index
12. Preparation of karyotype
Scheme of Evaluation
Time: 3 Hours                                                                                                                              Max. Marks 30
The scheme of evaluation at the end of session will be as under:
Two experiments              :   20 Marks Viva on experiments        :    5 Marks
Practical record                 :    5 Marks
CLASS XII (2018-19)
(THEORY) COURSE STRUCTURE
One Paper                                                                                                                            Max. Marks 70+30
Time: 3 hrs.
Units No. of Periods Marks
Unit V Protein and Gene Manipulation 100 40
Unit VI Cell Culture and Genetic Manipulation 80 30
Practicals 60 30
Total 240 100
One paper                                                                                                                                            Time: 3 hrs.
Total Marks : 70                  180 Periods
Unit-V Protein and Gene Manipulation                                                                  40 Marks     100 Periods
Chapter-1: Recombinant DNA Technology
Introduction, Tool of rDNA technology, Making rDNA, Introduction of recombinant DNA into host cells, Identification of Recombinants, Polymerase Chain Reaction (PCR), Hybridization Techniques, DNA Library, DNA Sequencing, Site-directed Mutagenesis
Chapter-2: Protein Structure and Engineering
Introduction to the world of proteins, 3-D shape of proteins, Structure-Function Relationship in Proteins, Purification of Proteins, Characterization of Proteins, Protein Based Products, Designing Proteins (Protein Engineering)
Chapter-3: Genomics, Proteomics and Bioinformatics
Introduction, Genome, Sequencing Projects, Gene prediction and counting, Genome Similarity, SNPs and Comparative Genomics, Functional Genomics, Proteomics, History of Bioinformatics, Sequences
and nomenclature, Information Sources, Analysis using Bioinformatics tools
Unit-VI Cell Culture and Genetic Manipulation                                                  30 Marks         80 Periods
Chapter-1: Microbial Cell Culture and its Applications
Introduction, Microbial Nutrition and Culture Techniques, Measurement and Kinetics of Microbial Growth,  Scale-up  of  Microbial  Process,  Isolation  of  Microbial  Products,  Strain  Isolation  and Improvement, Applications of Microbial Culture Technology, Biosafety Issues in Microbial Technology Chapter -2: Plant Cell Culture and Applications
Introduction,  Cell  and  Tissue  Culture  Techniques,  Applications  of  Cell  and  Tissue  Culture,  Gene Transfer Methods in Plants, Transgenic Plants with Beneficial Traits, Biosafety of Transgenic Plants
Chapter-3: Animal Cell Culture and Applications
Introduction, Animal Cell Culture Techniques, Characterization of Cell lines, Methods of Gene Delivery
into  Cells,  Scale-up  of  Animal  Culture  Process,  Applications  of  Animal  Cell  Culture,  Stem  Cell Technology, Tissue Engineering
PRACTICALS                                                                                                         30 Marks             60 Periods
Note: Every student will be required to do the following experiments during the academic session.
1. Use of special equipment in biotechnology experiments
2. Isolation of bacterial plasmid DNA
3. Detection of DNA by gel electrophoreses
4. Isolation of Genomic DNA (CTAB method)
5. Estimation of DNA
6. Bacterial transformation using any plasmid
7. Restriction digestion of plasmid DNA & its analysis by gel electrophoresis
8. Isolation of bacteria from curd & staining of bacteria
9. Cell viability assay
10. Data retrieval and data base search using internet site NCBI and download a DNA and protein sequence from internet, analyze it and comment on it
11. Reading of a DNA sequencing gel to arrive at the sequence
12. Project work
Scheme of Evaluation :
Time: 3 Hours                                                                                                                     Max. Marks 30
The scheme of evaluation at the end of the session will be as under:
A Two experiments 6+6 (only one computer based practical)
Practical record 04
Viva on Practicals 04
B Project work
Write up 05
Viva on project 05
Total 30
CBSE Prescribed Books:
1. A Text Book of Biotechnology – Class XI : Published by CBSE, New Delhi
2. A Laboratory Manual of Biotechnology – Class XI : Published by CBSE, New Delhi
3. A Text Book of Biotechnology – Class XII : Published by CBSE, New Delhi
4. A Laboratory Manual of Biotechnology – Class XII : Published by CBSE, New Delhi
BIO TECHNOLOGY (CODE – 045)
QUESTION PAPER DESIGN CLASS –XI & XII (2018–19)
Time 3 Hours                                                                                                                       Max. Marks: 70
S.
No.		 Typology of Questions Very Short Answer (VSA)
(1 mark)		 Short Answer-I (SA-I)
(2 marks)		 Short Answer-II (SA-II)
(3 marks)		 Long Answer (L.A.)
(5 marks)		 Total Marks %
Weightage
01 Knowledge Based 2 1 2 1 15 22%
02 Conceptual Understanding (Application and Reasoning based) 3 4 8 1 40 57%
03 Higher Order Thinking Skills (HOTS) 1 2 — — 05 07%
04 Skill Based — 1 1 1 10 14%
Total 6 8 11 3 70 100%
Total No. of questions = 28
1. No chapter wise weightage. Care to be taken to cover all the chapters.
2. The above template is only a sample. Suitable internal variations may be made for generating similar templates keeping the overall weightage to different form of questions and typology of
questions same.

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