LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

M.Sc. DEGREE EXAMINATION – BIO TECHNOLOGY

VE 34

SECOND SEMESTER – April 2009

BT 2819 – ENVIRONMENTAL BIOTECHNOLOGY

 

 

 

Date & Time: 24/04/2009 / 1:00 – 4:00  Dept. No.                                                   Max. : 100 Marks

 

 

                                                        PART – A                                              (20 Marks)

1. Choose the best answer: (5×1=5)

 

1. 1. The progressive accumulation of non-biodegradable chemicals through the food chain is known as
2. ecological balance b) trophic  structure
3. biological magnification d) biodegradation
4. Which of the following gas has maximum contribution to enhanced green house effect?
5. CFC b) CH₄                         c) N₂ O                        d) CO₂
6. The method of introducing natural microbial strains or GEMS to the contaminated site is called
7. a) Biostimulation b) Bioremediation
8. c) Bioaugmentation d) Bioaccumulation
9. If BOD of river is high, it means that the river is
10. a) not polluted b) highly polluted
11. c) gets least amount of light d) has low temperature of  water
12. Metallothioneins are rich in
13. a) Cysteine b)Leucine                         c)Proline                             d)Glutamic acid

 

1. State true or false. If false; give reasons: (5×1=5)

 

6. Fossil fuels are derived from long dead biological material.
7. Assimilable organic carbon fraction represents the easily degradable compounds.
8. Polyhydroxyalkanoates are biodegradable polymers.
9. The ideal microbe for bioleaching of metals from ore is Bacillus subtilis.
10. 10. Bacterial merA operon degrades inorganic mercury.

 

III. Complete the following:                                                                                     (5×1=5)

 

11. Compounds which persist in the environment are called ____________________.
12. Gasohol is a mixture of ____________________ and ______________________.
13. The world environment day is celebrated on _________________________.
14. Denaturing agents used in DGGE are____ ________ and ______________________.
15. Lin A gene degrades ________________________.

 

1. Answer the following, each in about 50 words: (5×1=5)

           

16. What is activated carbon?
17. Name the particulate pollutants found in atmosphere.
18. Differentiate Bioventing and Biosparging
19. Define Siderophores with examples.
20. What are Phase – I reactions?

 

 

 

 

 

PART – B

1. Answer any five of the following questions each in about 350 words: (5×8=40)

 

21. What are the effects of air pollution? Add a note on its remedial measures.
22. Write about the attached aerobic treatment processes with examples.
23. Describe the problems and remedies in soaps, surfactant and dye manufacturing industries.
24. “Microbes can convert waste into energy” – Justify the statement.
25. Write about the pathway involved in Toluene degradation by Pseudomonas putida.
26. Explain the process of Composting of organic waste.
27. Write about the role of Poplar and Arabidopsis as Phytoremediating plants.
28. Describe Biphenyl degradation by microbes.

 

PART – C

Answer the following each in about 1500 words:                                                  (2×20=40)

 

29. (a) What is remote sensing? Explain its working principle and applications.

(or)

(b) Explain the biofilm formation,  ecology and its control measures.

30. (a) Elaborate upon the procedures used by Dr Anand Chakrabarty in getting US patent

No 4259444.

(or)

(b)Write about T-RFLP as a tool for molecular analysis of pollutant degrading microbes.

 

 

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