LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

LM 36

M.Sc. DEGREE EXAMINATION – ZOOLOGY / BMIS

SECOND SEMESTER – APRIL 2007

CH 2901 – INSTRUMENTAL METHODS OF CHEMICAL ANALYSIS

 

 

 

 

Date & Time: 26/04/2007 / 1:00 – 4:00      Dept. No.                                       Max. : 100 Marks

 

 

Part-A   ANSWER ALL QUESTIONS (10 x 2 = 20)

 

1. Define: (i) molarity (ii) ppm
2. Explain Bathochromic shift with an example.
3. What is the volume of 6M H₂SO₄ (MW=98 gmol^-1) that contains 20 g of H₂SO₄ solute?
4. What are the two sources of flames used commonly in Atomic Absorption Spectrometers?
5. Define the specific rotation of an optically active compound.
6. Determine the pH of 10^-3M Ba(OH)₂
7. Compare the fundamental vibrational frequency of C-O and C=O.
8. How is the internuclear distance determined by X-ray diffraction studies?
9. Which of the following will give NMR signal (i) ₈O¹⁶ and (ii) ₆C¹³
10. Predict the m/e value for the molecular ion peak of C₆H₆.

 

 

Part-B   ANSWER ANY FIVE QUESTIONS (5 x8 = 40)

 

11. (a) State Beer-Lambert’s law and explain under what conditions it is not applicable.

(b) With a schematic diagram indicate the different parts and working of a Double-Beam Spectrophotometer. What are its advantages?                                      (3+5)

1. With a sketch discuss the principle, parts and working of a fluorimeter and how it is used in the analysis of riboflavin (vitamin B₂).

13. With a diagram outline the principle, parts and working of a cold-vapor atomic absorption spectrometer in the determination of mercury in a given biological sample?

1. With a schematic diagram discuss the principle, parts and working of a spectropolarimeter. Mention any one application.
2. How is the fluoride content in water sample estimated using ion selective electrode?
3. Describe the principle and instrumentation of NMR spectroscopy.
4. Write an account of ‘Isotopic dilution analysis’.
5. Calculate the fundamental frequency in the infrared absorption region for the C=C stretching vibration. (k = 9.77 x 10⁵ dynes cm^-1; c = 3 x 10¹⁰ cms^-1)

 

 

 

Part-C   ANSWER ANY TWO QUESTIONS (2 x20 = 40)

 

19. (a) Discuss in detail the instrumentation and application of nephelometry

 

(b) Discuss the principle and working of an Atomic Absorption Spectrometer

and how it can be used in the quantitative determination of lead (Pb²⁺) in blood.

(10+10)

20. Define and explain briefly the following concepts and its importance with a

suitable example:

 

(a) Difference Spectrometry (b) turbidimetry (c) ICP AES   (d) monochromator

(e) hollow-cathode lamp                                                                    (4 each)

 

20. (a) Discuss the principle and instrumentation of HPLC.

(b) Describe the applications of neutron diffraction.

 

21. (a) Give an account of the application of IR spectroscopy.

(b) Distinguish the following

H    H                                            H           H

|      |                                              |             |

(i)    H –C—C—O—H           and       H—C—O—C—H    using NMR.

|      |                                              |             |

H    H                                            H           H

H

|

(ii)   CH₃ –C—H           and           CH₃ –C–OH             using IR

||                                            |

O                                           H

 

 

(iii)   CH₃OH                 and          CH₃–CH₂–OH         using mass spectra.

 

 

 

 

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