LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
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M.Sc. DEGREE EXAMINATION – MEDICAL LAB. TECH.
SECOND SEMESTER – APRIL 2008
ML 2952 – ADVANCED LAB. TECHNIQUES
Date : 24/04/2008 Dept. No. Max. : 100 Marks
Time : 1:00 – 4:00
SECTION A
Answer ALL the questions: (10 x 2 = 20 Marks)
- What are the advantages of nucleic acid based identification methods?
- What is VNTR?
- Mention the characteristics of the different types of restriction endonucleases.
- What is homopolymer tailing?
- Define homologous chromosome.
- What is an allele?
- Define mutation.
- What is mosaicism?
- Mention the uses of DNA in forensic science.
- List the molecular techniques used in forensic investigation.
SECTION B
Answer any FOUR of the following (4 x 10 = 40 Marks)
- Write briefly on gel electrophoresis.
- Give the difference between southern, northern and western blotting techniques.
- Write a brief account on plasmids.
- How is cell culture done for karyotyping? Explain.
- Write short notes on Prader Willi syndrome and Angelman syndrome.
- Give a brief account on DNA databases.
SECTION C
Answer any TWO of the following (2 x 20 = 40 Marks)
- Write in detail about PCR technique.
- Explain in detail about the quality control and quality assurance in a molecular
diagnostic laboratory.
- Write in detail on structural chromosomal abnormalities.
- Explain in detail about Y chromosome analysis and Mitochondrial DNA analysis.
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