LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION – MEDICAL LAB. TECHNOLOGY
SECOND SEMESTER – APRIL 2011
ML 2954 – ADVANCED LAB.TECHNIQUES
Date : 12-04-2011 Dept. No. Max. : 100 Marks
Time : 1:00 – 4:00
Answer ALL the questions PART A 2×10=20 marks
- What is gene cloning?
- Define the role of restriction enzymes.
- What is paternity test?
- Differentiate sticky ends from blunt ends.
- What are transgenic animals?
- Define RFLP and RAPD.
- What are cosmids?
- Enumerate the solid supports used in electrophoresis.
- Define YAC and BAC.
- What is Immuno florescence?
Answer any FOUR PART B 4×10=40 marks
- Write notes on super vectors.
- Give an account on gel electrophortic separation.
- Describe in short northern blotting.
- Characterise plasmids.
- Explain ELISA.
- Write notes on gene mapping.
Answer any TWO PART C 2×20=40 marks
- Give a detailed account on microarray.
- Describe in detail significance of DNA in forensic science.
- Write an essay on gene therapy.
- Give an account of polymerase chain reaction.
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