LOYOLA COLLEGE (AUTONOMOUS), CHENNAI– 60034

M.Sc. DEGREE EXAMINATION  –     ZOOLOGY

FIRST  SEMESTER  – NOVEMBER 2003

ZO 1800/Z  717  histochemistry and microtechnique                               

04.11.2003                                                                                        Max: 100 Marks

1.00 – 4.00

 

PART – A                 (10 ´ 2 = 20 Marks)

Answer ALL the questions.  Each question carries TWO marks.

1. What is Freeze-drying?
2. What is Dioxane?
3. Expand: ICMR, DPX, STEM & PAS.
4. Differentiate Biopsy and Autopsy.
5. Comment on Photomicrography.
6. What is Keratin?
7. Differentiate Mordant and Lake?
8. What is Vital staining?
9. Distinguish between Honing and stropping.
10. Define Metachromasia?

PART – B                  (4 ´ 10 = 40 Marks)

Answer any FOUR questions.  Each question carries TEN marks.

11. Give an account of Ultramicrotomy.
12. Describe the Procedure adopted in the Dry Mount of insects.
13. Classify Proteins and give an account of Ninhydrin -Schiff Method.
14. Give an account on Cryostat and its advantages.
15. Describe the Procedure involved in extraction and mounting of soil

microarthropods.

16. Explain the identification and extraction of DNA by Feuigen Nuclear Reaction and Brach Method.

 

PART – C                 (2 ´ 20 = 40 Marks)

Answer any TWO questions.  Each question carries TWENTY marks.

17. What is Autoradiography and explain its applications in biology.
18. Explain the working principle of SEM and its sample preparation.
19. Describe the different steps involved in Paraffin Microtomy.
20. Write short notes on
21. Fluorescent Microscope
22. Classification of Lipids and its detection by Sudan Black B test.

 

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