LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION – ZOOLOGY
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FIRST SEMESTER – NOV 2006
ZO 1811 – HISTOCHEMISTRY & MICROTECHNIQUE
Date & Time : 04-11-2006/1.00-4.00 Dept. No. Max. : 100 Marks
PART – A
Answer ALL the questions. Each question carries TWO marks. (10 x 2 = 20 marks)
- What is freeze-drying?
- What is vital staining?
- What is biopsy?
- What is photomicrography?
- Comment on Metachromasia?
- What are Mordant and Lake?
- What is Dioxane?
- Distinguish between honing and stropping.
- What is collagen?
- Expand: ICMR & DPX.
PART – B
Answer any FOUR questions. Each Question carries TEN marks. (4 x 10 = 40 marks)
- Describe the steps involved in Ultra microtomy.
- Classify Proteins and describe Ninhydrin-Schiff method.
- Write an account on Cryostat and its advantages.
- Describe the procedure involved in Extraction and Mounting of soil microarthropods.
- Classify carbohydrates and its detection by PAS method.
- Describe the procedure adopted in the Dry Mount of insects.
PART – C
Answer any TWO questions. Each question carries TWENTY marks.
(2 x 20 = 40 marks)
- Describe Autoradiography and its applications.
- Explain the working principle of SEM and its sample preparation.
- Describe the different steps involved in Paraffin Microtomy.
- Write short notes on
- Classification of Lipids and its detection by Oil Red O method.
- Fluorescent Microscope and its applications.
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