LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION – ZOOLOGY
FIRST SEMESTER – NOVEMBER 2010
ZO 1817 – HISTOCHEMISTRY & MICROTECHNIQUE
Date : 11-11-10 Dept. No. Max. : 100 Marks
Time : 1:00 – 4:00
PART – A
Answer ALL the questions. Each question carries TWO marks. (10 x 2 = 20 marks)
- Comment on fixative.
- What is cytophotometry?
- Comment on biopsy.
- What is quenching?
- Comment on freeze drying.
- What is lake?
- Comment on dioxane.
- Distinguish honing from stropping.
- What is keratin?
- Expand: CPD and STEM.
PART – B
Answer any FOUR questions. Each question carries TEN marks. (4 x 10 = 40 marks)
- Explain the working principle of SEM.
- Describe the procedure involved in mounting of soil microarthropods.
- Write an account on cryostat and its advantages.
- Classify carbohydrates and its detection by PAS method.
- Give an account on insect kill bottle and pinning of insects.
- Describe the working principle of fluorescent microscope.
PART – C
Answer any TWO questions. Each question carries TWENTY marks. (2 x 20 = 40 marks)
- Describe autoradiography and its applications.
- Explain the procedure involved in specimen preparation for TEM.
- Give an account on the different steps involved in paraffin microtomy.
- Write short notes on:
- Classify proteins and describe Ninhydrin-Schiff method.
- Classify lipids and describe Oil Red O method.
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