LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

           B.Sc. DEGREE EXAMINATION – ADVANCED ZOOLOGY & BIOTECH.

 

DE 14

FIFTH SEMESTER – APRIL 2008

AZ 5502 – BIOTECHNOLOGY

 

 

 

Date : 30-04-08                  Dept. No.                                        Max. : 100 Marks

Time : 1:00 – 4:00

 

PART-A

Answer ALL the questions                                                                            (2 x 10 = 20 marks)

 

1. What is Cosmid?
2. What is SI Nuclease?
3. Comment on Super bug?
4. Write a note on Restriction Endonucleases & its type?
5. What is Palindromic Sequence?
6. What is Explant?
7. What is transfection?
8. Write a note on transgenic Fish?
9. Write a note on Biochips?
10. What is Bio transformation?

 

PART – B

Answer any FOUR questions                                                                        (4 x 10 = 40 marks)

 

1. Explain about the basic fermentor design and types of fermentor?
2. Give a brief account on gene cloning Vectors?
3. Write a short note on In-Vitro amplification using PCR types and applications?
4.  Write a short note on   a) Organo genetic method   b) Embryogenesis method
5. Give an account on Interferons, types and its uses in the field of Biotechnology.
6. Bring out the Socio-ethical problems of Biotechnology?

PART – C

Answer any TWO questions                                                                    (2 x 20 = 40 marks)

 

1. Discuss the Present challenges of Biotechnology in Agriculture and Environment?
2. Give a detail account on gene therapy and its Method.
3. Give a detailed account on Bio-sensors?
4. Write in detail about the steps involved in Construction and analysis of CDNA Clones?

 

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