LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

B.Sc. DEGREE EXAMINATION – CHEMISTRY & ADVAN. ZOOL.& BIO-TECHN.

VE 05

THIRD SEMESTER – April 2009

PB 3200 – GENERAL MICROBIOLOGY

 

 

 

Date & Time: 16/04/2009 / 1:00 – 4:00         Dept. No.                                                       Max. : 100 Marks

 

 

PART A

       Answer all questions                                                                                (20 Marks)

           

1. Choose the correct answer               (5×1 =5 Marks)

 

1. Which of the following is not true for fungi

a.heterotrophic         b.  saprophytic             c. parasitic                      d. autotrophic

2. Barophilic microorganisms are those which grow at
3. a. cold temperature high pressure
4. high temperature d. high pH values
5. Transcription and translation are related to
6. DNA replication b. glycolysis
7. protein synthesis d. the electron transport chain
8. Which of the following is not true for Mycoplasma?
9. pleomorphic b. membrane contains sterol
10. penicillin resistant             d. non- self reproducing
11. Name the enzyme which is a substitute for calf renin
12. alkaline protease b. acid protease
13. neutral protease d. lipase

 

 

II State whether the following statements are true or false                  (5×1= 5 Marks)

 

6. The phycobiliproteins are characteristic of green algae.
7. Bacterial flagella are composed of a carbohydrate known as peptidoglycan.
8. The bases adenine, guanine and cytosine are found in both RNA and DNA, but RNA

has thymine also.

9. TMV is sap transmissible and enters the host through wounds.
10. Malted barley is used in beer production.

 

 

 

 

III Complete the following                                                                             (5×1 = 5 Marks)

 

11. The reserve food material in fungi is ____________.
12. __________ microorganisms are those capable of living in different

environments.

13. The operon has been studied in depth in the common bacterium_________.
14. _____________ is grown in highly specified culture medium containing sterol.
15. The source for streptomycin is __________.

 

1. Answer all, each in about 50 words   (5x 1 = 5 Marks)
2. What are capsomeres?
3. Write short notes on inclusion bodies.
4. What is transformation?
5. Suggest any two measures to control tobacco mosaic disease.
6. Define sulphate reduction.

PART B

Answer any five, each within 350 words only.  Draw diagrams and flowcharts wherever

necessary.                                                                                          (5x 8= 40 Marks)

 

21. Discuss briefly about the general features of viruses.
22. Describe the classification of bacteria based on shapes and flagellation.
23. Explain the stages of microbial growth curve.
24. Describe the process of bacterial conjugation.
25. Give a brief account of citrus canker.
26. Write about the pathogen, symptoms and control measures of wheat rust disease.
27. Give an account of trickling filters.
28. Write notes on penicillin production.

PART C

Answer the following, each within 1500 words only.  Draw diagrams and flowcharts wherever necessary.                                                         (2×20= 40 Marks)

• Explain in detail about the following :

1. salient features of algae ii) salient features of fungi

                                                OR

1. Write notes on
2. i) Nitrogen cycle                               ii) Microbial enzymes

30 a. Explain the various pure culture techniques and add a note on maintenance of pure  cultures.

OR

1. Write an essay on DNA replication in E. coli.

 

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