LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
B.Sc.
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DEGREE EXAMINATION –CHEMISTRY
FIFTH SEMESTER – APRIL 2007
CH 5401 – BIO CHEMISTRY
Date & Time: 02/05/2007 / 1:00 – 4:00 Dept. No. Max. : 100 Marks
PART – A
Answer all the questions (10 ×2 = 20 marks)
- Define isoelectric point of an amino acid.
- Mention the components of the nucleus and give their functions.
- Define enzyme immobilization.
- What happens during rancidification?
- How is starch different from glycogen?
- What are sterols? Give an example.
- What is Sanger’s reagent? Mention its use.
- What are plasma proteins? Give an example.
- What are carbohydrates? How are they classified?
- Why DNA is the genetic material whereas RNA is not?
PART – B
Answer any eight questions (8 × 5 = 40 marks)
- a) What is meant by denaturation of proteins?
b) Give the mechanism of reaction of amino acids with ninhydrin. - Explain the structure and functions of the following organelles
a) cell wall b) golgi apparatus c) vacuole - Suggest a method of synthesis for the following amino acids
a) alanine b)methionine c) cysteine. - Explain the mechanism of enzyme catalyzed reaction using Lock and Key and Induced fit models.
- How are fats classified? Give suitable examples.
- Explain β-oxidation of fatty acids.
- Elucidate TCA cycle.
- Write note on the electron transport chain.
- Name the different types of RNA and list down their functions.
- ‘DNA replicates semi-conservatively’. Explain.
- What is a genetic code? Give its significance.
- How are phospholipids classified? Give suitable examples.
PART – C
Answer any four questions (4 × 10 = 40 marks)
- Explain the following
a) deamination b) transdeamination
c) transamination d) ornithine cycle - What are inhibitors? Explain competitive, non-competitive and allosteric inhibitions.
- a) How are fatty acids biosynthesized
b) What are phospholipids? How are they classified? - Explain glycolysis.
- a) Distinguish between animal and vegetable fats.
b) Give the various steps involved in oxidative phosphorylation. - How are proteins biosynthesized?