LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
B.Sc. DEGREE EXAMINATION – CHEMISTRY.
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THIRD SEMESTER – April 2009
PB 3204 – BIOINFORMATICS – I
Date & Time: 16/04/2009 / 1:00 – 4:00 Dept. No. Max. : 100 Marks
PART A
Answer all the questions (20 marks)
- Choose the best answer (5X1=5)
Answer all the questions
- Data model used in bioinformatics application is
- a) hierarchial b)relational c)network d)multidimensional
- NCBI hosts
- a) BLAST b) FASTA c)DDBJ d)Lalign
- Aromatic aminoacids are
- a) tyrosine and tryptophan
- b) tyrosine and threonine
- c) phenylalanine and alanine
- d) phenylalanine and leucine
- Double helical model of DNA was published by
- a) Watson and Crick
- b) Linus Pauling and Crick
- c) Rosalind Franklin and Crick
- d) Corey and Watson
- Addition of adenine to mRNA is called
- a) 3’ modification
- b) 5’ modification
- c) Poly A tailing
- d) Polymerase Chain reaction
- State whether the following true or false (5X1=5)
- Chargaff rule says A=T and G=C.
- Removal of introns and reunioin of exons is called Splicing.
- RefSeq is a standard database for comparative analyses.
- Cn3D is compatible with PDB.
10.NebCutter tool identifies restriction sites.
III. Complete the following (5X1=5)
- Proline and glycine are found in _______ of protein secondary structure.
- Proof reading function of polymerase is due to________ activity.
- PubChem Substance is a _________ database.
14._________ are produced by gene duplication within a species but has different functions.
- The latest genome sequenced is from an endangered species _______.
- Answer the following, each not exceeding 50 words. (5X1=5)
- Compute pI/MW
- Expand HSP
- What is a Start codon?
- Define DotPlot
- What is a Rooted Phylogenetic Tree?
PART B
(5X8=40)
Answer any five of the following, each not exceeding 350 words.
Draw diagrams and flowcharts wherever necessary.
- Explain OMIM and its features.
- What are the features of PDB?
- Discuss about BLAST and its types.
- How will you analyze the gene structure in eukaryotes using software tools?
- List the databases involved in Protein Domain analyses.
- Explain about proteomic tools.
- Detail the various molecular visualization tools with emphasis on Homology modeling.
- Write about ORF finder of NCBI.
PART C
Answer the following, each not exceeding 1500 words (2X20=40)
Draw diagrams and flowcharts wherever necessary.
29a) Explain Smith Waterman Local Algorithm by aligning the following sequences
ACG TAT CGC GTA TA
GAT GCT CTC GGA AA
Or
- b) Elaborate upon Multiple Sequence alignment and its software tools.
30 a) Write about the mechanism of translation of protein in detail.
Or
- b) How does the process of DNA replication occur inside the cell?