LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

ED 03

   B.Sc. DEGREE EXAMINATION – CHEMISTRY & ZOOLOGY

THIRD SEMESTER – November 2008

PB 3200 – GENERAL MICROBIOLOGY

 

 

 

Date : 13-11-08                     Dept. No.                                        Max. : 100 Marks

Time : 9:00 – 12:00

PART A

       Answer all questions                                                                              (20 Marks)                                

       Choose the correct answer                                                                (5×1 =5 Marks)

 

1. Which of the following is seen in all algae?
2. Chlorophyll a         b. Chlorophyll c  c.Chlorophyll b                d. fucoxanthin
3. Cryopreservation involves storage
4. at low temperature             b.  at optimum temperature
5. for long periods                  d. at very low temperature using liquid nitrogen
6. Lac operon in E.coli is an example of ________operon.
7. inducible                             b. positively regulated
8. repressible d. negative inducible
9. Okazaki fragments are used to elongate
10. the leading strand towards the replication fork
11. the lagging strand towards the replication fork
12. the leading strand away from the replication fork
13. the lagging strand away from the replication fork
14. Which bacterium is dominant in Trickling filters?
15. Pseudomonas                         b. Aeromonas
16. c. E. coli Zoogloea

 

II State whether the following statements are true or False                  (5×1= 5 Marks)

 

6. The conidiospores are sexual spores.
7. The generation time of a bacterial population is the time it takes for a bacterial population to double.
8. Competence is related towards transformation.
9. Xanthomonas citri is a rod shaped, monotrichous and anaerobic bacterium.
10. Water can be purified by exposure to UV.

 

 

 

 

 

 

III Complete the following                                                                           (5×1 = 5 Marks)

11. The cell wall of diatoms are made of ___________.
12. The bacteria which grow in the presence of minimum quantities of free oxygen are

known as __________.

13. During replication of DNA, the enzyme known as __________ attaches nucleotides

together to form the new DNA strand.

14. __________ is a heteroecious fungus with macrocyclic life cycle.
15. Desulfovibrio is involved in ______________.
16. Answer all, each in about 50 words   (5x 1 = 5 Marks)
17. Write short notes on enveloped viruses.
18. Distinguish between flagella and fimbriae.
19. Define transcription.
20. What is dikaryotisation?
21. What are vaccines?

PART B

Answer any five, each within 350 words only.  Draw diagrams and flowcharts wherever necessary.                                                                                                                 (5x 8= 40 Marks)

21. List out the salient features of algae.
22. What is a microbial colony? How can the numbers of bacteria be measured in a bacterial

population?

23. Explain the different methods of reproduction in bacteria.
24. Describe the structure and functioning of lac operon in E.coli.
25. Briefly write about Little leaf of brinjal.
26. Give a brief account of the Tobacco Mosaic disease.
27. Write short notes on microbial enzymes.
28. Discuss briefly about sulfur cycle.

PART C

Answer the following, each within 1500 words only.  Draw diagrams and flowcharts wherever necessary.                                                                                                      (2×20= 40 Marks)

 

29 a. Write detailed notes on the characteristic features and classification of viruses.

                                                      OR

1. Explain the following:
2. i) Cheese production ii) Beer production

 

30 a. Describe the ultrastructure of a bacterial cell.

OR

1. Explain the following:
2. i) Bacterial conjugation ii) Transduction

 

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