LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

M.Sc. DEGREE EXAMINATION – BIO TECHNOLOGY

VE 27

FIRST SEMESTER – April 2009

BT 1820 – ADVANCED BIOCHEMISTRY

 

 

 

Date & Time: 04/05/2009 / 1:00 – 4:00  Dept. No.                                                   Max. : 100 Marks

 

 

PART A                                             (20 marks)

1. Choose the best answer: (5 x 1 = 5)
2. In living system, most of the reactions fall into the category of

(i) Oxidation – reduction                    (ii) Internal rearrangement                              (iii) Group transfer                              (iv) All of those

2. Amino acid molecules can be covalently joined through a substituted amide linkage, termed as,

(i) Dipeptide    (ii) Covalent bond       (iii) Peptide bond        (iv) Isoelectric bond

3. The overall yield of ATP from the complete oxidation of one glucose molecule is

(i) 30 – 32        (ii) 20-24                     (iii) 16-19                    (iv) 6-9

4. The possible energy relationship between catabolic and anabolic pathway.

(i)  ATP → ADP                                 (ii) NADH→NAD                                          (iii) FADH₂ → FAD                           (iv) All of those

5. Hormones which are released into the blood and carried to target cells throughout the body are called

(i) Endocrine   (ii) Paracrine                (iii) Autocrine              (iv) None of these

1. State true or false, if false give the reason: (5 x 1 = 5)
2. The configuration of molecules is changed only by breaking bonds.
3. Sickle-cell anemia is a molecular disease of hemoglobin.
4. DNA isolated from different tissues of the same species has the same base composition.
5. The first step of glycolysis is reversible.
6. Lowered blood glucose triggers secretion of glucose of glucagons and increases insulin release.

III. Complete the following:                                                                             (5 x 1 = 5)

11. The energy required to break a bond is called …………..
12. Human erythrocytes are formed from precursor stem cell called …………..
13. The main storage polysaccharide of animal cell is ………….
14. The agents which completely scavenge the free radicals are known as ………….
15. The water soluble hormones epinephrine and norepinephrine are collectively termed as…………..

 

 

 

1. Answer the following each in about 50 words: (5 x 1 = 5)
2. Define Functional group. List out the functional groups of biomolecules.
3. Write the biological role of molecular chaperones.
4. Define: Mutarotation.
5. What are Apolipoproteins?
6. Mention the biological importance of hypothalamus.

 

PART B

1. Answer any five questions, each in about 350 words: (5 x 8 = 40)
2. Explain briefly the principles of bioenergetics.
3. Write in detail about the amino acid classification and physical properties.
4. Explain the role of water and fat soluble vitamins in living system with an example.
5. Write short notes on regulation and integration of metabolism.
6. Discuss the types of fatty acid oxidation and add a note on their importance.
7. Explain the principle and separating technique for electrophoresis.
8. How does the drug get metabolized and distributed in liver?
9. Explain the chemical nature and biological action of prostaglandin?

 

PART C

1. Answer the following each in about 1500 words: (2 x 20 = 40)
2. (a) Explain in detail the classification, structure, chemistry and properties of carbohydrates.

(OR)

(b) Give an account of glucose metabolism in blood.

30. (a) Explain the role of enzymes in determining the concentration of metabolites for clinical diagnosis.

(OR)

(b) Describe the biosynthesis of pancreatic hormone. Explain the regulation of insulin and glucagons.

 

 

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