LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

M.Sc. DEGREE EXAMINATION – BIO TECHNOLOGY

AF 01

SECOND SEMESTER – April 2009

BT 2815 – BIO-INSTRUMENTATION

 

 

 

Date & Time: 24/04/2009 / 1:00 – 4:00         Dept. No.                                                       Max. : 100 Marks

 

 

PART –A

Answer all the questions:                                                  (20 marks)

 

I  Choose the best answer:                                                      (5×1=5)

1 One curie is defined as

(a)  3.7x 10¹⁰disintegrations/ second       (b) 3.7x 10¹²disintegrations/ second

(c) 3.7x 10⁸disintegrations/ second         (d) 3.7x 10¹¹disintegrations/ second

2. Handerson Hasselbach equation is represented as

(a) pH= pK + log A^–/ HA                                (b) pH= pK + log HA/A^–

(c) pH= pK – log A^–/ HA                                 (d) pH= pK-log HA/A^–

3. The logarithm of the reciprocal of the transmittance is called as

(a) quantum efficiency (b) optical density (c) extinction (d) concentration

4. The volume of mobile phase present outside the gel particles is called as

(a) elution volume (b) bed volume (c) void volume (d) total volume

5. Which of the following is not included under the main text of the thesis?

(a) introduction (b) review (c) results (d) references

 

II Say true or false, if false state reason:                             (5×1=5)

 

6. In isopycnic centrifugation, separation is independent of buoyant density of the particle.
7. TEMED catalyses the formation of free radicals from ammonium per sulfate.
8. Avidin is not used as ligand for separation of biotin containing enzymes in affinity

chromatography.

9. 1-phenyl 4-phenyloxazole is used as secondary scintillant in liquid scintillation

counting

10. MEDLINE is an example of off-line literature search.

 

III Complete the following:                                                   (5×1=5)

 

11. ———– is the ratio of weight of the particle in the centrifugal field to the weight of

same particle when acted by gravity alone.

12. SDS-PAGE is used for the analysis of ———- and ———.
13. ————— is a helium consisting of two protons and two neutrons
14. A mixture of substances with different partition coefficients can be separated by a

technique called ———-

15. —————— is the hardest portion in any thesis.

 

 

 

 

 

 

 

IV Answer the following each within 50 words:         (5×1=5)

 

16. Compare prism and grating in UV-vis spectrophotometer.
17. Define isotachophoresis.
18. Define isocratic elution
19. What is quenching?
20. What is Byline?

 

 

PART-B

 

V Answer any 5 questions each within 350 words:                      (5×8=40)

 

21 Describe the principle and instrumentation of Atomic emission flame

spectrophotometry.

22 Write an account on principle and applications of Biosensors.

23. Explain the principle and procedure of ion exchange chromatography

24 Describe Geiger-Muller Counter with a diagram. Add a note on its applications with

biosafety measures adopted.

25. Explain the principle and procedure for the separation of isoenzymes

using polyacrylamide gel electrophoresis.

26. Write notes on principle and applications of Mass spectroscopy
27. Describe the principle and applications of radioimmunoassay.

28   What are the various points to be considered while writing a review paper?

 

 

PART-C

 

VI Answer any one of the following each within 1500 words:        (2×20=40)

 

29 (a) Explain the principle, design and applications of analytical ultracentrifuge. Add a

note on their advantages over conventional centrifuges.

(Or)

(b) Write the principle, operation and applications of Rank oxygen electrode.

30 (a) Describe in detail about HPLC and its operation with a flow diagram.

(Or)

(b) Explain the various parts of a thesis in detail, giving the significance of each

portion.

 

 

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