LOYOLA COLLEGE (AUTONOMOUS), CHENNAI– 60034
M.Sc. DEGREE EXAMINATION – ZOOLOGY
FIRST SEMESTER – NOVEMBER 2003
ZO 1800/Z 717 histochemistry and microtechnique
04.11.2003 Max: 100 Marks
1.00 – 4.00
PART – A (10 ´ 2 = 20 Marks)
Answer ALL the questions. Each question carries TWO marks.
- What is Freeze-drying?
- What is Dioxane?
- Expand: ICMR, DPX, STEM & PAS.
- Differentiate Biopsy and Autopsy.
- Comment on Photomicrography.
- What is Keratin?
- Differentiate Mordant and Lake?
- What is Vital staining?
- Distinguish between Honing and stropping.
- Define Metachromasia?
PART – B (4 ´ 10 = 40 Marks)
Answer any FOUR questions. Each question carries TEN marks.
- Give an account of Ultramicrotomy.
- Describe the Procedure adopted in the Dry Mount of insects.
- Classify Proteins and give an account of Ninhydrin -Schiff Method.
- Give an account on Cryostat and its advantages.
- Describe the Procedure involved in extraction and mounting of soil
microarthropods.
- Explain the identification and extraction of DNA by Feuigen Nuclear Reaction and Brach Method.
PART – C (2 ´ 20 = 40 Marks)
Answer any TWO questions. Each question carries TWENTY marks.
- What is Autoradiography and explain its applications in biology.
- Explain the working principle of SEM and its sample preparation.
- Describe the different steps involved in Paraffin Microtomy.
- Write short notes on
- Fluorescent Microscope
- Classification of Lipids and its detection by Sudan Black B test.