LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

M.Sc. DEGREE EXAMINATION – BIO TECHNOLOGY

VE 46

FOURTH SEMESTER – April 2009

BT 4951 – METABOLIC ENGINEERING

 

 

 

Date & Time: 23/04/2009 / 9:00 – 12:00  Dept. No.                                                    Max. : 100 Marks

 

 

 

SECTION A  (20 marks)

Answer all the questions

 

1. Choose the best answer                                     (5X1=5)

 

1. Energy required to express the house keeping genes are derived from
2. a) Biosynthetic reaction c) Fueling reaction
3. b) Enzymatic reaction d) Hormonal reaction

 

2. Initial rate of an enzyme catalysed reaction depends on
3. a) Enzyme concentration c) pH of the medium
4. b) Substrate concentration d) Temperature of the medium.

 

3. A ligand can be a
4. a) Protein c) Carbohydrate
5. b) Lipid d) metal ion

 

4. Choose the carbon source which enters the TCA cycle.
5. a) Glucose b) Acetyl coA c) Pyruvate                  d) Citrate

 

5. Name the enzyme for which insulin is not an inducer
6. a) Phosphofructokinase c) Glucose-6-Phosphatase
7. b) Pyruvate kinase d) Glycogen synthetase

 

1. State True or False; if false give reasons (5X1=5)

 

6. Primary metabolism synthesizes building block metabolite and energy in uniform

amounts.

7. The fluxes in and out of the Black box model are given by a constant rate and a

variable substrate and product rate.

8. Recombinant PCR cannot be used for making novel proteins.
9. Strongly weak nodes are insensitive to regulation.
10. Secondary metabolites cannot be overproduced by altering flux.

 

III. Complete the following.                                                             (5X1=5)

 

11. NLS corresponds to _______.
12. Glutamic acid was overproduced in_______.
13. Hsp refers to________.
14. Electronic cell mimicks ________.
15. GOGAT stands for________.

 

 

1. Answer the following, each about 50 words (5X1=5)

 

16. Explain Substrate level phosphorylation.
17. What is an irreversible inhibitor?
18. Write about UAS.
19. Define Metabolic Control theory.
20. Explain Gene shuffling.

SECTION B

 

 Answer any five, each answer within 350 words only.                (5X8=40)

 

21. What are perturbations? How are they helpful in analyzing flux?
22. Describe Pentose phosphate pathway and its regulation.
23. Give a schematic representation of various types of enzyme inhibition.
24. What are secondary metabolites? Discuss its production in a plant cell.
25. What are the criteria to be employed in the choice of an organism for metabolite

overproduction?

26. Give a blueprint for the selection of induced mutants synthesizing ethanol from xylose.
27. What are the potentials and perils of Metabolic engineering?
28. Discuss the glycolysis pathway and its regulation in Corynebaterium glutamicum.

overproducing Lysine.

 

SECTION C

 

Answer the following, each in about 1500 words.                          (2X20=40)

 

29 a) Elaborate the classification of nodes and highlight the various strategies adopted for

accelerating primary metabolism.

(Or)

1. b) Outline the pathway of TCA cycle and MFA of a plant cell.

 

30 a) Give a detailed description on Protein Engineering and discuss the various methodologies

followed.

 

(Or)

1. b) Write about Signal Transduction and the pathways involved.

 

 

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