LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

M.Sc. DEGREE EXAMINATION – BIO TECHNOLOGY

VE 40

SECOND SEMESTER – April 2009

BT 2954 – MICROBIAL & BIOPROCESS TECHNOLOGY

 

 

 

Date & Time: 29/04/2009 / 1:00 – 4:00  Dept. No.                                                  Max. : 100 Marks

 

 

                                                            PART – A                                          (20 Marks)

1. Choose the best answer: (5×1=5)
2. Which one of the following is a secondary metabolite?
3. a) proteins b) lipids                                   c) nucleotides              d) antibiotics
4. Name the fermenter part that is used to check vortex and improve aeration?
5. a) agitator b) ball-bearing             c) sparger                     d) baffles
6. The evaporator used for concentrating higher viscous product is
7. a) falling-film b) plate evaporator      c) forced-film              d) centrifugal-forced film
8. The CO₂ concentration is commonly monitored using
9. a) paramagnetic gas analyser b) galvanic electrode
10. c) infra-red gas analyser d) polarographic electrode
11. Which one of the following is not used in cell disruption?
12. a) sonicator b) French press                        c) lyophiliser                d) homogenizer

 

1. State true or false. If false; give the reason: (5×1=5)
2. Enriched cultures are ideal for isolating industrially important organisms.
3. The two-phase aqueous separation is induced when hydrophilic polymers are added.
4. In inoculum development, the age of inoculum is important for the growth of sporulating bacteria.
5. In batch fermentation, the substrate is added at the start of the fermentation.
6. Sodium lauryl sulphate is a solvent.

 

III. Complete the following:                                                                                     (5×1=5)

11. The advantage of using stainless-steel in the fermentor as construction material is _____________

and _____________________.

12. The process of inducing multiple mutations at the replication point is called ________________.
13. The measure of electron activity is called __________________________.
14. The chromatographic separation based on molecular recognition is ________________________.
15. The separation of components from a liquid mixture by treatment with a solvent is known as

________________________.

 

1. Answer the following, each in about 50 words: (5×1=5)
2. Define ideal Bioreactor.
3. What are precursors? Give examples.
4. What do you mean by interfacing -technique?
5. Name two enzymes used to disrupt yeast cells?
6. What is GMP?

PART- B

1. Answer any five of the following questions each in about 350 words: (5×8=40)
2. Explain briefly the ranges of fermentation processes.
3. What is sterilization? What are the methods used for sterilizing thermolabile substances?
4. Describe the brewing process of malted barley in beer production.
5. What is scale-up operation? Write about their significance in bioprocessing.
6. Describe the methods involved in cell-disruption.
7. Explain the computer aided control of fermentation.
8. Describe the solid-liquid separation in down-stream processing.
9. Discuss the ethical implications in bioprocessing.

                                                                        PART- C

1. Answer the following questions each in about 1500 words: (2×20=40)
2. (a) Comment on Single-cell protein production from bacteria and algae and their commercial

significance.

(or)

(b) Discuss the processes of strain improvement using mutation and recombination.

30. (a) Describe the effluent treatment and disposal in sewered areas.

(or)  

(b) Give an account of media formulation for bioprocessing.

 

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