LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & BIOTECHNOLOGY

AE 06

FIFTH SEMESTER – NOV 2006

         PB 5400 – MICROBIAL TECHNOLOGY

(Also equivalent to PBB 400)

 

 

Date & Time : 03-11-2006/9.00-12.00         Dept. No.                                                       Max. : 100 Marks

 

 

Part A

 

Answer all the questions                                                                     (20 marks)

 

1. Choose the correct answer (5 x 1 = 5 )

 

1. ­­_____ is used both as a carbon source and nitrogen source
2. a) Sucrose b) Molassus
3. c) Whey d) Soybean meal
4. Beer production does not involve
5. a) carbonation b) distillation c) filtration                   d) clarification
6. Auxotrophic mutants of yeast are used in the production of
7. a) Hepatitis B vaccine b) Erythopoietin c) Interferon α             d) Insulin
8. Which of the following is used in the production of acetic acid?
9. a) Glucanobacter b) Aspergillus c) Bacillus                    d) Penicillium
10. Azotobacter is used in the production of
11. a) biopesticide b) biofertilizer c) biosorban                d) biopolymer

 

1. State whether the following are True or False (5 x 1 = 5 )

 

6. Biosensors are used for online monitoring.
7. The protein content of Spirulina is 80%.
8. Streptomycin is a peptide containing antibiotic.
9. Xylanase can be used in bleaching of paper.
10. Mycorrhizae help to increase the uptake of phosphorus.

 

III. Complete the following                                                                   (5 x 1 = 5)

 

11. Turbulance in a fermentor is controlled using ________.
12. Hops is the female cone of _________ _________.
13. CNBr is used to cleave the peptide at ________ residues.
14. Glucanobacter is used for the production of __________________.
15. Dextran is produced using ______.

 

 

 

 

 

1. Answer the following in about 50 words (5 x 1 = 5)

 

16. Define cellular yield coefficient.
17. What is yogurt?
18. Mention any four microbes used in production of Riboflavin.
19. What is aspartame?
20. Define gasohols.

 

 

Part B

 

Answer any five of the following each in about 350 words. Draw necessary diagrams.

(5 x 8 = 40)

21. Give an account of the range of products obtained from microbe-based industries.
22. Write notes on the carbon sources used in formulating media.
23. Elaborate the steps involved in beer production.
24. Briefly discuss about the production of Spirulina and its importance.
25. Give an account of bioconversion.
26. List out the various industrially important enzymes, their sources and applications.
27. Explain the steps involved in production of citric acid.
28. What are biopolymers? Explain the source and uses of any four

 

Part C

 

Answer the following questions in about 1500 words. Draw necessary diagrams

(2 x 20 = 40)

 

29. a) Elucidate the structure and functioning of a typical fermentor. List out the salient features of an

ideal fermentor.

 

(or)

 

1. Give a detailed account of cheese production. Add a note on their types.

 

30. a) Explain: a) Production of Penicillin      b) Production of Hepatitis B vaccine.

 

(or)

 

1. What are biofertilizers? Give a detailed account of the types of biofertilizers and their applications.

 

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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

IB 29

B.Sc.  DEGREE EXAMINATION –PLANT BIOLOGY & PLANT BIO-TECH.

FIFTH SEMESTER – APRIL 2007

PB 5400 – MICROBIAL TECHNOLOGY

 

 

Date & Time: 03/05/2007 / 1:00 – 4:00          Dept. No.                                                     Max. : 100 Marks

 

 

PART A

 

1. Choose the correct answer (5 x 1 = 5 )

 

 

1. ­­Cultures can be preserved at _____ °C in cryopreservation.
2. a) -160 b) -100 c) -196                         b) -120
3. Saccharomyces is not used in the production of
4. a) Wine b) Champagne c) Hep B vaccine         d) Riboflavin
5. Which of the following is used in the production of Vitamin B2?
6. a) Saccharomyces b) E. coli c) Propionibacterium d) Vibrio         
7. Which of the following is used in the production of citric acid?
8. a) Glucanobacter b) Penicillium c) Aspergillus              d) Bacillus
9. Methanogens are is used in the production of
10. a) biogas b) biofertilizer. c) biopesticide.                        d) biopolymer

 

 

 

1. State whether the following are True or False (5 x 1 = 5 )

 

 

6. Ion excghange chromatography is used for product recovery.
7. Yoghurt is produced using
8. Erythropoitin can be produced by recombinant DNA technology.
9. Pectinase is used in cheese industries.
10. Dextran is obtained from

 

 

 

III. Complete the following                                                                 (5 x 1 = 5)

 

 

11. N-Alkanes are used as _______ source
12. The DNA content of Spirulina is ______%
13. Ashbya gossypii is used for the production of _______.
14. The chemical name for Aspartame is __________________.
15. Baculoviruses are used as ________

 

 

 

 

 

 

 

 

 

 

 

 

1. Answer the following in about 50 words (5 x 1 = 5)

 

16. Mention the functions of sparger and baffles.
17. What are single cell proteins? Give examples.
18. List any four microbes used in production of Vitamin B12.
19. What is rennet? Mention its uses.
20. What are exopolymers? Give examples.

 

 

PART B

 

Answer any five of the following each in about 350 words. Draw necessary diagrams.  (5 x 8 = 40)

 

 

21. Discuss about the different nitrogen sources used in the formulation of medium.
22. Explain the steps involved in directed mutagenesis for strain improvement.
23. Write notes on Beer production.
24. Give an account of humulin production.
25. Explain in detail about the production of ß- carotene.
26. Briefly explain about amylase production using microbes.
27. Discuss in brief about the production of Vinegar.
28. Write notes on biopesticides and their production. Add note on its merits.

 

PART C

 

Answer the following questions in about 1500 words. Draw necessary diagrams         (2 x 20 = 40)

 

29. a) Explain in detail about methods used in production of recombinants for improved industrial

strains.

 

(or)

 

1. Give a detailed account of cheese production. Add a note on the types of cheese.

 

30. a) Explain: a) Production of Streptomycin  b) Biotransformation.

 

(or)

 

1. i. Elaborate processes involved in the production of citric acid.
2. Write notes on Exopolysaccarides and their applications

 

 

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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

ED 19

   B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.

FIFTH SEMESTER – November 2008

PB 5400 – MICROBIAL TECHNOLOGY

 

 

 

Date : 12-11-08                     Dept. No.                                        Max. : 100 Marks

Time : 9:00 – 12:00

 

PART A (20 marks)

Answer all questions

 

1. Choose the correct answer (5 x 1 = 5)

 

1. Fermentors are constructed using
2. a) glass b) steel                         c) titanium                   d) all the above
3. Whey is used as a source for _____
4. a) carbon b) nitrogen                   c) vitamin                    d) inducer
5. Hepatitis B vaccine is administered to immunize against
6. a) typhoid b) cholera                    c) jaundice                   d) TB
7. Acetic acid is obtained from
8. a) Glucanobacter b) Pseudomonas          c) Aspergillus              d) Alcaligenes
9. The source for dextran is
10. a) Pseudomonas b) Leuconostoc                        c) Acetobacter             d) Vibrio

 

1. State whether the following statements are true or false (5 x 1 = 5)

 

6. Baffles increase aeration.
7. Fusarium is used in the production of mycoprotein.
8. Insulin is a dipeptide hormone.
9. Blakeslea is used in ß-carotene production.
10. Xanthan is a polysaccharide from fungi.

 

 

III. Complete the following                                                                (5 x 1  = 5 )

 

11. Soyabean meal is used popularly as a _____ source.
12. Spirulina is a _______ alga.
13. Vitamin B6 is produced by the microorganism ______.
14. Rennet is a _______ protease.
15. Alginate is obtained from the bacterium ______.

 

 

1. Answer the following each in about 50 words (5 x 1= 5)

 

16. Define spargers.
17. Mention any two lactic acid producing bacteria.
18. Name two organism used in the production of vitamin A.
19. Define immobilization.
20. What is gasohol?

 

PART  B

 

Answer any five of the following, each answer not exceeding 350 words

Draw diagrams and flowcharts wherever necessary                           ( 5 x 8 = 40)

 

21. Write short notes on the characteristics of a medium.
22. List out the facilities needed in a fermentation based industry.
23. Write short notes on cheese production.
24. Describe the process involved in cultivation of Spirulina.
25. Write notes on Penicillin production.
26. Explain in detail about the production of Vitamin B12.
27. Discuss about the production of citric acid.
28. Write notes on biopesticides.

 

PART C

 

Answer the following, each answer not exceeding 1500 words.

Draw diagrams and flowcharts wherever necessary                           (2 x 20 = 40)

 

29. Describe in detail about the structure of a fermentor and explain the function of each part.

 

(or)

1. Write detailed notes on Beer production.

 

30. Write notes on : i. Insulin production ii. Biotransformation

(or)

1. What are biofertilizers? Explain the methods used in the mass production of any two biofertilizers.

 

 

 

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