LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & BIOTECHNOLOGY

AE 08

FIFTH SEMESTER – NOV 2006

PB 5403 – PLANT BIO-CHEMISTRY

 

 

Date & Time : 06-11-2006/9.00-12.00  Dept. No.                                                       Max. : 100 Marks

 

 

 

Part A                                           (20 marks)

Answer All questions

 

I Choose the Correct Answer                                                                     ( 5 x 1 = 5 marks)

 

1. Solution having pH 6 is …….
2. acidic b. highly acidic            c. alkaline                    d. highly alkaline.
3. Which of the following one belongs to ketone group.
4. Dihydroxyacetone b. Glucose       c. Pentose        d. Fructose
5. Mention the amino acid having symmetric α – carbon.
6. Glycine b. Serine          c. Methionine              d. Cystine
7. Pick the odd one out.
8. Fats b. Waxes         c. Glycolipids              d. Terpenoids
9. Nomenclature of an enzyme is assigned with …… numbers.
10. 1                       b. 2                  c. 3                  d. 4

 

 

II State whether the following statements are  True or False    ( 5 x 1 = 5 marks)

6. The pH of the blood higher than the normal condition is called alkalosis.
7. Polysaccharides are sweet in taste.
8. Globular proteins are soluble in water.
9. Saturated fatty acids do not contain double bond.
10. Enzyme with organic molecule is called coenzyme.

III. Complete the following                                                                     ( 5 x 1 = 5 marks)

11. The working principle of a colorimeter is based on …. and ……… law.
12. Covalent linkage between monomers of a polysaccharide is called ……….
13. The R group in Glycine is …..
14. Competitive inhibitor for succinic acid is …..
15. Fatty acids with one double bond are known as …………

 

1. Answer all, each in about 50 words ( 5 x 1 = 5 marks)
2. Expand SDS – PAGE.
3. What is inulin?
4. Mention any two aromatic amino acids.
5. What is lecithin?
   20. Define V- max.

 

 

PART B                       ( 5 x 8 = 40 marks)

 

Answer any five, each within 350 words only. Draw diagrams and flowcharts wherever necessary.

 

21. Define biomolecule. Describe the structure of an atom.
22. What is a bond? Explain ionic, covalent, coordinate and glycosidic bond.
23. Describe the classification of amino acids.
24. Write notes on sucrose and maltose in plants.
25. Give an account of classification of proteins.
26. Define holoenzymes. Explain the various factors affecting the enzyme activity.
27. Explain the types of secondary metabolites. Add a note on their significances.
28. What is Michalies – Menton constant? Explain the enzyme kinetics and Km value .

 

PART C                       ( 2 x 20 = 40 marks)

 

Answer the following, each within 1500 words only. Draw diagrams and flowcharts wherever necessary.

 

29. a) Explain the working principle and design of any four-biochemical techniques.

 

Or

 

1. b) Write an essay on general classification, structure and properties of carbohydrates.

 

30. a) Describe the various structure of a protein. Enumerate the biological role of

peptides.

 

Or

 

1. b) Give an account of:
2.   classification of lipids and fatty acids. ( 12 marks)
3. oxidation of fatty acids.. (8 marks)

 

 

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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

IB 27

B.Sc  DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.

FIFTH SEMESTER – APRIL 2007

PB 5403 – PLANT BIO-CHEMISTRY

 

 

Date & Time: 04/05/2007 / 1:00 – 4:00          Dept. No.                                                     Max. : 100 Marks

 

 

 

Part A                                           (20 marks)

Answer All questions

 

I Choose the Correct Answer                                                                     ( 5 x 1 = 5 marks)

1. The pH denotes the relative concentration of …… ions in a solution.
2. hydrogen b. hydroxyl                  c. oxygen         d. both a and b

 

2. Pick the odd one out.
3. Starch b. Cellulose                 c. Glycogen     d. Glucose

 

3. The number of peptide bond present in a tripeptide is ….
4. 1 b. 2                  c. 3                  d. 4

 

4. Name the additional group present in Phospholipids.
5. phosphoric acid b. sulphuric acid          c. lecithin         d. citric acid

 

5. Mention the site at which substrate binds with an enzyme.
6. active site b. N-terminal               c. Carboxyl end           d. both b and c

 

 

II State whether the following statements are  True or False    ( 5 x 1 = 5 marks)

6. TEMED acts as catalyst in SDS-PAGE.
7. Sucrose is the condensation of two molecules of fructose.
8. The R group in Glycine is hydrogen.
9. An alcohol group in lecithin is glycerol.
10. Enzyme involved in oxidation-reduction reaction is called oxidoreductase.

 

III. Complete the following                                                                     ( 5 x 1 = 5 marks)

11. ……….. force is involved in centrifugation.
12. The reserved carbohydrate in cell wall is …….
13. Tryptophan is a …………. amino acid.
14. Esters of fatty acids with other than glycerol is called as ……..
15. Km refers to ………..

 

1. Answer all, each in about 50 words ( 5 x 1 = 5 marks)

 

16. Expand TLC and GLC.
17. Define Disaccharide.
18. What is β – sheet structure?

 

19. Define glycolipid.

 

20. What is active site?

PART B                       ( 5 x 8 = 40 marks)

Answer any five, each within 350 words only. Draw diagrams and flowcharts wherever necessary.

 

21. Define biomolecule. Explain how chemical bonding occurs in biomolecules.
22. Explain the working principle of centrifuge. Add a note on various types of

centrifuge.

23. Describe the structure and properties of cellulose and starch.
24. Briefly explain the structure of protein.
25. Give an account of classification of amino acids based on R group.
26. Explain the mode of action of enzymes.
27. Write notes on saturated and unsaturated fatty acids.
28. Describe the classification of enzymes.

PART C                       ( 2 x 20 = 40 marks)

 

Answer the following, each within 1500  words only. Draw diagrams and flowcharts wherever necessary.

 

29a.  Give a detailed account of different supporting media used in  electrophoresis.

 

Or

1. Explain the structure and properties of monosaccharide and disaccharides.

 

30a. Give an account on the classification and properties of protein.

 

Or

 

1. i.  What is an enzyme? Describe the general classification and enzyme kinetics.
2. Explain the types of enzyme inhibitors.

 

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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

   B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.

ED 10

FIFTH SEMESTER – November 2008

PB 5512 – PLANT BIO-TECHNOLOGY

 

 

 

Date : 10-11-08                     Dept. No.                                        Max. : 100 Marks

Time : 9:00 – 12:00

 

PART –A                                       (20 marks)

 

1. Choose the correct answer (5×1=5)

1. The sugar used in MS medium is

1. Sucrose b. Maltose c. Galactose       d. Glucose

 

1. The fusogen used in protoplast fusion  is

1. PEP b. PEG c. KNO₃         d. PVP

 

1. The type of DNA present in the chloroplast is

1. ss linear DNA b ds linear DNA   c. ds circular DNA    d. ss circular DNA

 

1. The tracking dye used in electrophoresis is

1. Bromophenol blue b. Bromocresol blue
2. Bromothymol blue d. Bromoazol blue

 

1. The organism used as bioinsecticides for cotton is

1. Bacillus thruemgensis b. Bacillus polymyxa
2. Bacillus subtilis d. Bacillus cereus

 

1. State whether the following statements are True or False (5×1=5)

 

1. Cytokinins are the hormones used for shoot induction.

 

1. nod genes are present in the legume nodules.

 

1. Somatic embryo can be initiated by absisic acid.

 

1. Meristem is considered as the region free of microbes.

 

1. Western Blotting is used for DNA separation.

 

III. Complete the following                                                                      (5×1=5)

 

1. ______________ is an unorganised mass of cells.

 

1. RAPD can be expanded as ______________________________.

 

1. Cryopreservation is carried out at ____________°C temperature.

 

1. Ti plasmid is responsible for production of the hormones like______________.

 

1. The enzyme used in PCR is_______________ polymerase.

 

IV Answer the following within 50 words                                       (5×1=5)

     Write short notes on

 

1. IBA

 

1.  Somaclonal variation

 

1. Embryo culture

 

1. Particle gun bombardment

 

1. Transgenic plants

 

PART – B

Answer any FIVE of the following in about 350 words                       (5×8=40)

 

1. Write the procedure followed for induction of shoot and root.
2. Explain somatic hybridisation.
3. Describe the mitochondrial genome and its function.
4. Describe electrophoresis of RNA
5. Explain the concept of molecular probes.
6. Elaborate on selection markers and their uses.
7. Describe the scope and history of Plant Tissue culture.
8. What are Ti plasmids? Explain the molecular mechanism involved in transformation                       with Ti plasmid.

 

PART-C

Answer the following in about 1500 words                                          (2X20=40)

 

29. a. Write in detail the in vitro culture of plants using hormone.

OR

1. Describe organ culture and add a note on artificial seeds.

 

1. a. Explain the use of gene mapping and molecular markers in crop improvement.

OR

1. Discuss in detail the organisation of plant genome, mitochondrial DNA

and chloroplast |DNA.

                                                 

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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

ED 21

   B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.

FIFTH SEMESTER – November 2008

PB 5403 – PLANT BIO-CHEMISTRY

 

 

 

Date : 14-11-08                     Dept. No.                                        Max. : 100 Marks

Time : 9:00 – 12:00

PART A                                                   (20 marks)

Answer All questions

 

I Choose the Correct Answer                                                                     ( 5 x 1 = 5 marks)

1. Solution having pH 6.5 is …….
2. low acidic b. highly acidic            c. low alkaline             d. highly alkaline.
3. The α – carbon in all amino acids is asymmetric except ….
4. proline b. serine                       c. glycine                     d. glutamine
5. The number of peptide bonds present in a tripeptide is ….
6. 1 b. 2                  c. 3                  d. 4
7. Pick the odd one out.
8. fats b. waxes          c. glycolipids               d. terpenoids
9. The multiple forms of an enzyme catalyzing the same reaction is ………
10. holoenzyme b. isoenzyme               c. co enzyme               d. allosteric enzyme

 

II State whether the following statements are True or False               \  (5 x 1 = 5 marks)

6. TEMED acts as a catalyst in SDS-PAGE.
7. Starch is a homopolysaccharide.
8. Globular proteins are soluble in water.
9. An alcohol group in lecithin is glycerol.
10. IUB refers to international union of biochemistry.

 

III. Complete the following                                                                     ( 5 x 1 = 5 marks)

11. Transfer of one or more electrons from one atom to another is referred to as …..
12. A carbon is said to be asymmetric when it is attached to …. different atoms or groups.
13. The R group in Glycine is …..
14. ………. fatty acids do not contain double bond.
15. ……….. catalyzes bond formation between two substrate molecules.

 

1. Answer all, each in about 50 words  ( 5 x 1 = 5 marks)
2. What is Beer and Lambert law?
3. What is inulin?
4. Distinguish between essential and non-essential amino acids.
5. Define fatty acid.
6. What is a coenzyme?

 

PART B                       (5 x 8 = 40 marks)

Answer any five, each within 350 words only. Draw diagrams and flowcharts wherever necessary.

 

21. Write down the basic principle and types of chromatography. Add a note on its significance.
22. Define biomolecules. Describe the structure of an atom.
23. Describe the structure and properties of cellulose and starch.
24. What biological functions do proteins perform?
25. Give an account of classification of proteins.
26. Explain the types of secondary metabolites. Add a note on their significance
27. Describe the classification of enzymes.
28. Give a brief account of oxidation of fatty acids

PART C                       (2 x 20 = 40 marks)

 

Answer the following, each within 1500 words only. Draw diagrams and flowcharts wherever necessary.

 

29a. Give a detailed account of SDS-PAGE and Agarose gel electrophoresis.

 

Or

1. Explain the structure and properties of monosaccharide and disaccharides.

 

30a. i . Define active site.  Explain the mechanism of enzyme action (8 marks).

1. Define enzyme inhibition. Explain the types of enzyme inhibition. (12 marks)

Or

1. i. Describe the general classification of lipids and fatty acids.(10 marks)
2. Briefly explain the chemical bonds involved in protein structure. (10 marks)

 

 

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