LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

M.Sc. DEGREE EXAMINATION – BIO TECHNOLOGY

VE 32

SECOND SEMESTER – April 2009

BT 2817 – RECOMBINANT DNA & GENE CLONING

 

 

 

Date & Time: 20/04/2009 / 1:00 – 4:00  Dept. No.                                                       Max. : 100 Marks

 

 

                                    PART – A                                          (20 Marks)

1. Choose the best answer: (5×1=5)
2. The site on DNA at which RNA polymerase binds on the DNA to precede transcription is called
3. a) primer b) promoter                 c) operator                   d) repressor
4. Which one of the following is an artificial plasmid?
5. a) Col E1 b) RSF  1030               c) pSC 101                  d) pBR 322
6. The process involving direct uptake of naked DNA is called
7. a) transformation b) transduction                        c)transfection              d) recombination
8. M 13 phage can produce
9. a) SS DNA b) DS DNA                 c) both SS & DS DNA’s        d) either  SS or DS DNA’s
10. Which one of the following is a selectable marker gene?
11. a) npt II b) Lux                         c) Gus                          d) GFP

 

1. State true or false. If false; state the reason: (5×1=5)
2. Ligation is the formation of phosphodiester bonds.
3. Lambda gt1 1 contain lac Z gene with a unique EcoRI site.
4. The red and gam gene products are used in lac selection.
5. The Lac Z¹ gene has multiple cloning sites.
6. S₁ nuclease removes the single strands protrusions at each end.

 

III. Complete the following:                                                                                     (5×1=5)

11. Cloning all DNA fragments of an organism at a time in a population of a bacterium is called

______________________.

12. The EcoRI restriction enzyme produces ___________________ ends.
13. The chain termination method of DNA sequencing is also called ______________________.
14. The insitu – hybridization assay is also called _______________________.
15. Polyhedrin gene is isolated from ______________________ virus.
16. Answer the following questions each in about 50 words: (5×1=5)
17. What is X – gal?
18. Define shuttle vectors.
19. What is fusion protein?
20. Distinguish insertional and replacement vectors.
21. What is Ti – plasmid?

PART – B

1. Answer any five of the following questions each in about 350 words: (5×8=40)
2. Distinguish between selectable and scorable markers.
3. Explain insertional mutagenesis, with labelled sketch.
4. Describe the southern blotting technique.
5. Explain the principle and working methodology of Real – time PCR.
6. Elaborate how the baculoviruses are useful in biotechnology and write its applications.
7. Describe the chemical and biological methods of transformation.
8. Describe the lifecycle of M 13 phages and its role as a vector.
9. Give an account on chain termination sequencing.

                                               

PART – C

1. Answer the following, each in about 1500 words: (2×20=40)
2. (a) What are restriction endonucleases? Describe their types and properties with examples.

(or)                             

(b) Describe the various genetic markers used in the selection of recombinant clones.

30. (a) Give an account on Yeast artificial Chromosomes. Add a note how YAC are advantageous than

other vectors used in cloning.

(or)

(b) What is a cDNA library? Describe the steps involved in the synthesis of cDNA.

 

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