LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION – ADV. ZOOLOGY & BIO TECH.
FOURTH SEMESTER – APRIL 2011
ZO 4811 – BIOTECHNOLOGY
Date : 09-04-2011 Dept. No. Max. : 100 Marks
Time : 9:00 – 12:00
PART – A ( 10 x2=20)
ANSWER ALL IN A FEW SENTENCES
- Distinguish southern blotting and northern blotting techniques.
- Name any fouir restriction enzymes.
- Illustrate Ti plasmid.
- Differentiate genomic DNA froncomplimentary DNA.
- State the principle involved in the production of polyclonal antibodies.
6.What are the limitations of GMOs?
7.What are xenobiotics ? Mention their importance.
- What are the demerits of SCP ?
- What are “Pharm animals” ?
10 Expand : a) RFLP b) ICSI
PART – B ( 4 x 10 = 40)
BRIEFLY ANSWER ANY FOUR
- What are the steps involved in transformation process ?
- Describe a method of DNA sequencing.
13.Critically analyse the rationale of IPP and IPR.
- Propose a protocol for cytotoxicity assaying of culture cells/organs.
- Examine the principle and applications of microarray technology.
- How do you relate organic farming and biofertilizers input ?
PART – C ( 2 x20=40)
ANSWER ANY TWO IN DETAIL
- Elucidate any four gene cloning vectors with a note on their applications.
- Examine enzyme technology in terms of production, purification and
applications.
19 How do you produce transgenic animals ? Discuss its merits and demerits.
20.Comment on: a) IVF b) Gene therapy c) Microinjection d) DNA computing.