LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

M.Sc. DEGREE EXAMINATION – BIO TECHNOLOGY

SECOND SEMESTER – April 2009

BT 2818 – ENZYMOLOGY & ENZYME TECHNOLOGY

Date & Time: 22/04/2009 / 1:00 – 4:00       Dept. No.                                                       Max. : 100 Marks

PART A                                                  (20 Marks)

1. Choose the best answer: (5 X 1 = 5)
2. Energy required by the reactants to undergo the reactions is known as:

(i) Input energy                                   (ii) Energy change                                                       (iii) Activation energy                         (iv) Enthalpy

2. Specificity of enzyme mostly depends on the:

(i) Apoenzyme            (ii) Coenzyme              (iii) Substrate               (iv) Reaction

3. Coenzyme not related to B complex which is required for glycogen synthesis is:

(i) ATP                        (ii) CDP                       (iii) UDP                     (iv) PAPS

4. The enzyme which plays a major role in Myocardial Infarction is:

(i) AST                        (ii) ALT                       (iii) LDH                     (iv) ACP

5. The mutant, which plays a major role in the mechanism of α-helix capping is:

(i) A4T                        (ii) G147A                   (iii) G189A                  (iv) K305D

1. State True or False: If false give the reason: (5 X 1 = 5)
2. Name of the enzyme usually indicates the substrate involved in a particular reaction.
3. Synthases and synthatases are different class of enzymes.
4. Coenzymes are otherwise called as second substrates.
5. Transition state analogs are potential enzyme inhibitors.
6. In endergonic reactions, the energy is liberated.

III. Complete the following:                                                                            (5 X 1 = 5)

11. Racemase and epimerase are the examples for the …………… class of enzyme.
12. The site at which both the ……………. and……….. are present is called as active site.
13. Isoenzymes are distinguishable only by ………………..
14. Lysozyme breaks the ……………….. linkages in Glucosaminoglycans.
15. Inhibitor responsible for the competitive inhibition closely resembles the …………..

1. Answer the following, each within 50 words:                  (5 X 1 = 5)
2. Define – Katal.
3. What is Turnover number?
4. Define: Enzyme degradation.
5. Explain: Enzyme electrode.
6. Define – Abzymes.

PART – B

1. Answer any five questions, each within 350 words: (5 X 8 = 40)
2. Explain in detail about the IUBMB classification of enzymes.
3. Discuss on importance and methods for isolation and purification of enzymes.
4. Write in detail about the types of reversible inhibition?
5. Define multienzyme complex. Explain evolutionary relationship between serine protease.
6. Explain the diagnostic importance of SGOT, SGPT and ALP in hepatic diseases?
7. Write about enzyme therapy for genetic disorders and cancer.
8. Explain in detail about the single point mutagenesis for optimization of industrial enzymes.
9. What are proteolytic enzymes? Explain their role in digestive tract.

PART – C

1. Answer the following, each answer within 1500 words: (2 X 20 = 40)
2. (a) Explain the specificity, salient features and mechanism of action of active sites.

(OR)

(b) Derive Michaelis-Menten equation. Explain the effect of substrate, temperature, pH, and product concentration on the regulation of enzyme activity.

30. (a) Explain the Phillip’s mechanism of enzyme action with reference to lysozyme. Give the importance of catalytic residues in serine proteases.

(OR)

(b) Explain the types, advantages and applications of enzyme immobilization with suitable examples.

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        LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

M.Sc. DEGREE EXAMINATION – BIO TECHNOLOGY

SECOND SEMESTER – April 2009

BT 2819 – ENVIRONMENTAL BIOTECHNOLOGY

Date & Time: 24/04/2009 / 1:00 – 4:00  Dept. No.                                                   Max. : 100 Marks

                                                        PART – A                                              (20 Marks)

1. Choose the best answer: (5×1=5)

1. 1. The progressive accumulation of non-biodegradable chemicals through the food chain is known as
2. ecological balance b) trophic  structure
3. biological magnification d) biodegradation
4. Which of the following gas has maximum contribution to enhanced green house effect?
5. CFC b) CH₄                         c) N₂ O                        d) CO₂
6. The method of introducing natural microbial strains or GEMS to the contaminated site is called
7. a) Biostimulation b) Bioremediation
8. c) Bioaugmentation d) Bioaccumulation
9. If BOD of river is high, it means that the river is
10. a) not polluted b) highly polluted
11. c) gets least amount of light d) has low temperature of  water
12. Metallothioneins are rich in
13. a) Cysteine b)Leucine                         c)Proline                             d)Glutamic acid

1. State true or false. If false; give reasons: (5×1=5)

6. Fossil fuels are derived from long dead biological material.
7. Assimilable organic carbon fraction represents the easily degradable compounds.
8. Polyhydroxyalkanoates are biodegradable polymers.
9. The ideal microbe for bioleaching of metals from ore is Bacillus subtilis.
10. 10. Bacterial merA operon degrades inorganic mercury.

III. Complete the following:                                                                                     (5×1=5)

11. Compounds which persist in the environment are called ____________________.
12. Gasohol is a mixture of ____________________ and ______________________.
13. The world environment day is celebrated on _________________________.
14. Denaturing agents used in DGGE are____ ________ and ______________________.
15. Lin A gene degrades ________________________.

1. Answer the following, each in about 50 words: (5×1=5)

16. What is activated carbon?
17. Name the particulate pollutants found in atmosphere.
18. Differentiate Bioventing and Biosparging
19. Define Siderophores with examples.
20. What are Phase – I reactions?

PART – B

1. Answer any five of the following questions each in about 350 words: (5×8=40)

21. What are the effects of air pollution? Add a note on its remedial measures.
22. Write about the attached aerobic treatment processes with examples.
23. Describe the problems and remedies in soaps, surfactant and dye manufacturing industries.
24. “Microbes can convert waste into energy” – Justify the statement.
25. Write about the pathway involved in Toluene degradation by Pseudomonas putida.
26. Explain the process of Composting of organic waste.
27. Write about the role of Poplar and Arabidopsis as Phytoremediating plants.
28. Describe Biphenyl degradation by microbes.

PART – C

Answer the following each in about 1500 words:                                                  (2×20=40)

29. (a) What is remote sensing? Explain its working principle and applications.

(or)

(b) Explain the biofilm formation,  ecology and its control measures.

30. (a) Elaborate upon the procedures used by Dr Anand Chakrabarty in getting US patent

No 4259444.

(or)

(b)Write about T-RFLP as a tool for molecular analysis of pollutant degrading microbes.

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