LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

M.Sc. DEGREE EXAMINATION – MEDICAL LAB. TECHNOLOGY

BV 10

SECOND SEMESTER – April 2009

ML 2954 / 2952 – ADVANCED LAB.TECHNIQUES

 

 

 

Date & Time: 24/04/2009 / 1:00 – 4:00   Dept. No.                                                    Max. : 100 Marks

 

 

SECTION A

Answer ALL the questions in one or two sentences:                                 (10 X 2 = 20 marks)

1. What is CODIS?
2. How can DNA be used to identify an individual?
3. List the factors affecting southern blotting.
4. What are the three steps in AmFLP?
5. Define allele.
6. What is a primer?
7. Draw the structure of a chromosome.
8. What abnormalities can be detected by cytogenetics?
9. Give examples of how DNA can be used in forensic identification.
10. What is STR analysis?

SECTION B

Answer any FOUR of the following:                                                          (4  X 10 = 40 marks)

11. What is Y chromosome analysis? How is it useful in genealogy?
12. Write short notes on RFLP.
13. Explain the methods of isolation and purification of nucleic acids.
14. Explain structural abnormalities of chromosomes with diagram.
15. Discuss briefly quality control and quality assurance in a molecular diagnostic laboratory.
16. Elaborate northern, southern and western blotting.

SECTION C

Answer any TWO of the following:                                                            (2 X 20 = 40 marks)

17. Discuss in detail chromosomes preparations for diagnosis.
18. How is DNA fingerprinting done? Explain.
19. Explain in detail the PCR technique. Add a note on its applications.
20. Write an essay on in situ