LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

M.Sc. DEGREE EXAMINATION – MEDICAL LAB. TECH.

HP 01

FOURTH SEMESTER – APRIL 2007

ML 4807 – HISTOPATHOLOGY AND ESSENTIALS OF LAB

 

 

 

Date & Time: 18/04/2007 / 9:00 – 12:00          Dept. No.                                                          Max. : 100 Marks

 

SECTION-A

 

Answer all the Questions:                             (10 x 2 = 20 Marks)

 

1. What are infiltration and impregnation?
2. How can mineralized bone be softened for sectioning.
3. What is PAP smear?
4. What is meant by vacuum embedding?
5. Define: a) Honing b) Stropping.
6. Write the preparation and use of egg albumin.
7. Mention different types of biopsy.
8. What is amyloidosis?
9. Comment on decalcification.
10. Mention phenyl and Indole group of amino acids.

SECTION-B

 

Answer any four of the following:               (4 x 10 = 40 Marks)

 

11. Write a short note on mucopolysaccharides and DMAB staining.
12. Give an account of cryostat sectioning.
13. Explain the disulfide and sulfhydryl groups of amino acids.
14. Write the principle, procedure and histological importance of methyl

green-pyronin method.

15. What is exfoliative cytology? Explain preparation and fixation of cytological

specimen.

16. Enumerate the problems encountered during section cutting.

 

SECTION-C

 

Answer any two of the following:                             ( 2 x 20 = 40 Marks)

 

17. Write an essay on different types of fixatives.
18. What is glycosaminoglycans? Explain the principle, procedure and histological

importance of PAS staining.

19. Write short notes on : a) Nile blue sulfate stain.
20. b) Freezing microtome.
21. Describe in detail any three-lipid identification methods.

 

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