LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

M.Sc. DEGREE EXAMINATION – MEDICAL LAB. TECH.

LM 17

FOURTH SEMESTER – APRIL 2008

ML 4807 – HISTOPATHOLOGY AND ESSENTIALS OF LAB

 

 

 

Date : 21/04/2008            Dept. No.                                        Max. : 100 Marks

Time : 9:00 – 12:00

 

SECTION-A

Answer ALL the questions:                                                                           (10 X 2=20 Marks)

 

1. Write the composition of formal saline fixative.
2. How will you prepare 650 ml of 0.9N hydrochloric acid solution?
3. What is exfoliative cytology?
4. What is cone Biopsy?
5. List the factors involved in fixation process.
6. What is a frozen section?
7. What is ambient temperature?
8. Define the terms Honing and stropping.
9. Write the composition and use of Mayer’s egg albumin.
10. Name the endogenous and exogenous pigments present in the tissue sample.

 

SECTION-B

Answer any FOUR of the following:                                                          (4 X 10=40 Marks)

 

11. Write short notes on different types of embedding media.
12. Describe in detail the decalcification process with suitable example.
13. How will you identify disulfide and indole group amino acids present in the tissue sample.
14. Write short notes on problems encountered during section cutting.
15. Write the principle, procedure and hitopathological importance of Sudan block B stain and

Toluidin blue stain.

16. Give a brief account on the different types of microtome knife with suitable diagram.

SECTION-C

Answer any TWO of the following:                                                            (2 X 20=40 Marks)

 

17. Describe the processing of histological tissues for paraffin embedding.
18. Write in detail the different types of microtomes.
19. Explain in detail the different staining methods involved in demonstration of nucleic acids.
20. Give an account on the collection, processing and staining of cytological specimen.

 

 

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