LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

M.Sc. DEGREE EXAMINATION – MEDICAL LAB TECHNOLOGY

HZ 9

FOURTH SEMESTER – APRIL 2006

                                                            ML 4951 – TISSUE CULTURE

 

 

Date & Time : 22-04-2006/9.00-12.00         Dept. No.                                                       Max. : 100 Marks

 

 

Part A                                     (Answer All)                         10 X 2 = 20

1. What is single cell culture?
2. What is the possible mechanism leading to somoclonal variation?
3. Classify suspension culture.
4. Give two examples of secondary cell lines.
5. What are the cryopreservatives used to preserve cells?
6. What type of instruments is used for plant tissue culture?
7. Give the basic composition of a plant tissue culture medium
8. What are the different categories of organ culture?
9. What is micropropagation?
10. Define somatic hybridization.

Part B                                    (Answer any four)              4 X 10 = 40

1. Explain leaf culture.
2. Give a general account of the expression of totipotency of cells in a culture.
3. Discuss the importance of artificial seeds
4. How is the growth of virus detected in cell culture?
5. What are cell lines? Explain the different types.
6. Briefly discuss lymphocyte separation.

 

Part C                                    (Answer any two)                           2 X 20 = 40

17. Describe the principle & application of root culture techniques mentioning the protocol for root culture.
18. Discuss the different types of protoplast fusion
19. What are phytohormones? Discuss its importance.
20. Describe the methodology & Importance of anther and pollen culture.

 

 

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