DE 22

LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

M.Sc. DEGREE EXAMINATION – ZOOLOGY

FIRST SEMESTER – APRIL 2008

ZO 1811 – HISTOCHEMISTRY & MICROTECHNIQUE

 

 

 

Date : 06-05-08                  Dept. No.                                        Max. : 100 Marks

Time : 1:00 – 4:00

PART – A

Answer ALL the questions. Each question carries TWO marks.                       (10 x 2 = 20 Marks)

1. Comment on vital staining.
2. What is photomicrography?
3. What is collagen?
4. What are Mordants and Lakes?
5. What is Metachromasia?
6. Comment on autopsy.
7. Comment on stoke shift.
8. Write a note on Mayer’s albumen.
9. Differentiate preservative from fixative.
10. Expand: ICMR& STEM.

PART – B

Answer any FOUR questions. Each question carries TEN marks.       (4 x 10 = 40 Marks)

11. Give an account on Fluorescent microscope and its applications.
12. Describe the procedure involved in dry mount of insects.
13. Classify lipids and describe Oil Red O method.
14. Give an account on Cryostat and its applications.
15. Describe the procedure involved in mounting of soil microarthropods.
16. Give an account on the identification of DNA by Feulgen reaction.

 

PART – C

Answer any TWO questions. Each question carries TWENTY marks.           (2 x 20 = 40 Marks)

17. What is Ultramicrotome? Describe the steps involved in ultramicrotomy.
18. Give an account on Autoradiography and its applications.
19. Explain the working principle of SEM and its sample preparation.
20. Write notes on:
    1. Classification of proteins and Ninhydrin-Schiff method.
    2. Explain the different steps of Haemotoxylin – Eosin staining procedure involved in Paraffin microtomy.

 

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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

GA 20

M.Sc. DEGREE EXAMINATION – ZOOLOGY

FIRST SEMESTER – November 2008

    ZO 1811 – HISTOCHEMISTRY & MICROTECHNIQUE

 

 

 

Date : 13-11-08                 Dept. No.                                        Max. : 100 Marks

Time : 1:00 – 4:00

PART – A

Answer ALL the questions                                                                                  (10 x 2 = 20 marks)

1. Comment on infiltration.
2. What is polymerization?
3. Comment on necropsy?
4. What is photomicrography?
5. Define metachromasia?
6. Comment on dioxane.
7. Comment on dehydration.
8. Differentiate SEM from TEM.
9. What is keratin?
10. Expand DPX and CPD.

 PART – B

Answer any FOUR questions                                                                            (4 x 10 = 40 marks)

11. Classify lipids and describe Sudan Black B method.
12. Give an account on Cryostat and its advantages.
13. Describe the procedure in involved in DNA identification and its extraction.
14. Explain the procedure involved in extraction and mounting of soil microarthropods.
15. Give an account on the procedure involved in dry mounting of insects.
16. Describe the working principle of Scanning Electron Microscope.

 

PART – C

 

Answer any TWO questions                                                                              (2 x 20 = 40 marks)

17. Classify carbohydrates and describe PAS method.
18. Describe Radio Active Labeling and its uses.
19. Explain the preparative steps in tissue processing for Ultramicrotomy.
20. What is microtomy? Explain the different steps involved in Paraffin Microtomy.

 

 

 

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