LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.
FIFTH SEMESTER – NOVEMBER 2010
PB 5518/PB 5512/PB 5504 – PLANT BIOTECHNOLOGY
Date : 09-11-10 Dept. No. Max. : 100 Marks
Time : 9:00 – 12:00
PART – A (20 Marks)
- Choose and circle the best answers of the following: (5 x 1= 5)
- Biotechnology can be defined as
(a) Science and technology (b) Biology and technology
(c) Production of a product from biological organisms using technology
(d) Production of a product using different technology from plants and animals
- Early attempts to derive callus, scientist used
(a) Tobacco pith cells and carrot cambial cells (b) Cauliflower
(c) Shoot tip of tobacco (d) Carrot cambial cells
- Virus free plants can be derived using
- Embryo culture (b) Mericulture (c) Anther Culture (d) Leaf disc culture
- The most conventional and natural method for gene transfer is by
(a) Biolistic gun (b) Microinjection (c) Agrbacterium mediated (d) Electroporation
- GUS gene used in DNA technology is
(a) Marker gene (b) Molecular marker (c) Silence gene (d) Jumping gene
- State whether the statements are TRUE or FALSE. (5 x 1 = 5)
- Sodium Hypochloride is a surface sterilising agent.
- Immobilised cells are called as synthetic seeds.
- Anabaena is a soil bacteria used as vector in Bt cotton.
- Taq DNA polymerase cleaves deoxyribonucleic acid.
- QTL is one the target for crop improvement.
III. Complete the following (5 x 1 = 5)
- Determination of cell growth by turbidity in a suspension culture system is known as
_________.
- A ring like vascular structure that develops inside callus is called as __________.
- Agrabacterium tumifaciens when causes Crown gall disease in plant the T-DNA triggers
the production of ____________ and ___________.
- Most important enzyme that is used in the construction of cDNA library is _______.
- Most important molecular tool in Crop Improvement studies is _____________.
- Answer the following, each within 50 words only. (5 x 1 = 5)
- What is an “explants”?
- Define Caulogenesis
- What is nif gene?
- Explain gene library
- Expand GMO? Give an example.
PART – B
Answer the following, each answer within 500 words. Draw diagrams and flowcharts
wherever necessary. (5 x 7 =35 Marks)
- a) Write the historical mile stone of tissue culture in brief.
(OR)
- b) Write short notes on components of tissue culture media.
- a) Describe how synthetic seeds are produced.
(OR)
- b) Give short account on Anther culture.
- a) How Rhizobium interacts with legume plants at the molecular level.
(OR)
- b) Write short notes on organization and function of Plant nuclear genome
- a) Define restriction enzyme. Explain its use in recombinant DNA technology
(OR)
- b) Explain the process of Polymerase Chain Reaction in gene amplification
- a) What are various biotechnological strategies applied for crop improvement.
(OR)
- b) Write the role of molecular markers in crop improvement.
PART – C
Answer any three of the following, each with 1200 words. Draw diagrams and flowcharts
wherever necessary. (3 x 15 = 45 Marks)
- Give a descriptive account on callus and cell suspension culture techniques.
- How protoplast is isolated, fused/manipulated and cultured.
- Describe how Agrobaterium is used in gene transfer.
- What are the different methods by which genes are directly transferred in to plants?
Describe methodology involved in Particle gun bombardment method.
- Write a detailed protocol to produce insect and herbicide resistant transgenic plants.
Latest Govt Job & Exam Updates: