LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
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B.Sc. DEGREE EXAMINATION – CHEMISTRY & ZOOLOGY
THIRD SEMESTER – November 2008
PB 3200 – GENERAL MICROBIOLOGY
Date : 13-11-08 Dept. No. Max. : 100 Marks
Time : 9:00 – 12:00
PART A
Answer all questions (20 Marks)
Choose the correct answer (5×1 =5 Marks)
- Which of the following is seen in all algae?
- Chlorophyll a b. Chlorophyll c c.Chlorophyll b d. fucoxanthin
- Cryopreservation involves storage
- at low temperature b. at optimum temperature
- for long periods d. at very low temperature using liquid nitrogen
- Lac operon in E.coli is an example of ________operon.
- inducible b. positively regulated
- repressible d. negative inducible
- Okazaki fragments are used to elongate
- the leading strand towards the replication fork
- the lagging strand towards the replication fork
- the leading strand away from the replication fork
- the lagging strand away from the replication fork
- Which bacterium is dominant in Trickling filters?
- Pseudomonas b. Aeromonas
- c. E. coli Zoogloea
II State whether the following statements are true or False (5×1= 5 Marks)
- The conidiospores are sexual spores.
- The generation time of a bacterial population is the time it takes for a bacterial population to double.
- Competence is related towards transformation.
- Xanthomonas citri is a rod shaped, monotrichous and anaerobic bacterium.
- Water can be purified by exposure to UV.
III Complete the following (5×1 = 5 Marks)
- The cell wall of diatoms are made of ___________.
- The bacteria which grow in the presence of minimum quantities of free oxygen are
known as __________.
- During replication of DNA, the enzyme known as __________ attaches nucleotides
together to form the new DNA strand.
- __________ is a heteroecious fungus with macrocyclic life cycle.
- Desulfovibrio is involved in ______________.
- Answer all, each in about 50 words (5x 1 = 5 Marks)
- Write short notes on enveloped viruses.
- Distinguish between flagella and fimbriae.
- Define transcription.
- What is dikaryotisation?
- What are vaccines?
PART B
Answer any five, each within 350 words only. Draw diagrams and flowcharts wherever necessary. (5x 8= 40 Marks)
- List out the salient features of algae.
- What is a microbial colony? How can the numbers of bacteria be measured in a bacterial
population?
- Explain the different methods of reproduction in bacteria.
- Describe the structure and functioning of lac operon in E.coli.
- Briefly write about Little leaf of brinjal.
- Give a brief account of the Tobacco Mosaic disease.
- Write short notes on microbial enzymes.
- Discuss briefly about sulfur cycle.
PART C
Answer the following, each within 1500 words only. Draw diagrams and flowcharts wherever necessary. (2×20= 40 Marks)
29 a. Write detailed notes on the characteristic features and classification of viruses.
OR
- Explain the following:
- i) Cheese production ii) Beer production
30 a. Describe the ultrastructure of a bacterial cell.
OR
- Explain the following:
- i) Bacterial conjugation ii) Transduction
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