LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

B.Sc.  DEGREE EXAMINATION –PLANT BIOLOGY & PLANT BIO-TECH.

IB 23

SIXTH SEMESTER – APRIL 2007

PB 6606 – FERMENTATION TECHNOLOGY

 

 

Date & Time: 20/04/2007 / 9:00 – 12:00       Dept. No.                                                                 Max. : 100 Marks

 

 

Part A

Answer all questions

 

1. Choose the best answer (5 x 1 = 5)
2. Microbe mediated conversion of a compound into a structurally related one is called
3. microbial transformation b. compound transformation
4. biotransformation d. molecular transformation

 

2. ____ = μ_maxs / (K_s + s)
3. ½ μ b. ½ μ_max c. μ²     d. μ

 

3. ______ is required to achieve a number of mixing objectives during fermentation.
4. Agitator b. Impeller c. Agitator or impeller d. Acetator

 

4. Measurement and control of dissolved oxygen is accomplished using _____ electrodes.
5. polarographic b. reference c. Bourdon tube          d. tubing

 

5. Reversible exchange of ions between a liquid phase and a solid phase is referred to as
6. ion-exchange b. ion-transfer c. ion-reversion            d. ion-mobilisation

 

1. State whether the following statements are true or false (5 x 1 = 5)
2. Baffles are incorporated into the fermenter to prevent vortex and to improve aeration efficiency.
3. In continuous culture systems, D = F / W.
4. During fermentation, monitoring equipment produces information to indicate the progress of the process.
5. In computer administered fermentation, data reduction is performed by the data analysis system.

 

III. Complete the following                                                                          (5 x 1 =5)

11. ______ technology has revolutionised and extended the production of several potential fermentation products.

 

12. In microbial growth kinetics, the maintenance phase is equivalent to _______ phase.

 

13. ________ fermenters are used highly for the production of Single Cell Proteins.

 

14. Carbon & Energy source + Nitrogen source + O₂ + other requirements → Biomass + _____ + CO₂ + H₂O + ∆.

 

15. ________ sensor is not an integral part of the fermenter.

 

 

 

1. Answer the following, each within 50 words (5 x 1 = 5)
2. Define fermentation in strict biochemical sense.
3. What is fed – batch culture?
4. Distinguish between asepsis and containment.
5. Functionally differentiate In-line and On-line sensors.
6. What are antifoams?

Part B

 

Answer any five of the following, each within 350 words                                                 (5 x 8 = 40)

21. Comment on the range of fermentation process.
22. Enumerate the basic functions of a fermenter.
23. Describe in brief the methods of preservation of industrially important microorganisms.
24. Give an account of the control systems of process variables in fermentation process.
25. Derive for the kinetics of batch fermentation.
26. Give the schematic representation of down stream processing with a brief explanation.
27. Write down the various applications of Biosensors.
28. Comment on the following fermentation vessels:
29. Waldhof – type fermenter ii.  Tower fermenter

iii. Cylindro – Conical vessels                   iv. Air – lift fermenters

 

Part C

 

Answer the following, each within 1500 words. Draw necessary diagrams

(2 x 20 = 40)

29 (a) Trace the chronological development of the fermentation industry.

(OR)

(b) Describe in general the preparation of media for industrial fermentation.

30 (a) Discuss the application of computers in fermentation technology.

(OR)

(b) Bring out the application of chromatography in fermented product recovery and

purification.

 

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