LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.

IB 31

FIFTH SEMESTER – APRIL 2007

PB 5504 – PLANT BIOTECHNOLOGY

 

 

 

Date & Time: 30/04/2007 / 1:00 – 4:00         Dept. No.                                                       Max. : 100 Marks

 

 

Part A                                           (20 marks)

Answer All questions

I Choose the Correct Answer                                                                     ( 5 x 1 = 5 marks)

 

1. Which one of the following media is most commonly used in plant tissue culture?
2. Whites medium b. B5 medium    c. Nitsch medium     d. M.S. medium

 

2. What term is given to the ability of a cell regenerated into a plantlet?
3. callus b. totipotency              c. meristem                  d. explant

 

3. A small fragment of radio-labeled DNA molecule complementary to the foreign

DNA is called…

1. cDNA b. DNA probe             c. palindromic DNA   d. T- DNA

 

4. Function of nod gene is ….
5. nitrogen fixation b. nodule development
6. nitrogen assimilation d. nitrogenase production

 

5. The purified form of agar is ……
6. cellulose b. agarose                    c. alginate                    d. sucrose

 

II State whether the following statements are True or False                (5 x 1 = 5 marks)

 

6. Laminar air flow cabinet consists of HEPA filter.
7. Knudson medium is used for orchid culture.
8. The opine synthesized by Nop. Ti plasmid is acetosyringone.
9. pBR 322 is constructed from pUC.
10. Sodium alginate is used as fusogent.

 

III.  Complete the following                                                                    ( 5 x 1 = 5 marks)

11. Hot air oven is used for …………. of glassware.
12. PEG refers to …………
13. Agrobacterium tumefasciens causes a disease called ………
14. The function of APS in Electrophoresis is ………
15. Spirulina contains ……… pigment.

 

 

 

 

1. Answer all, each in about 50 words ( 5 x 1 = 5 marks)

 

16. Define Micropropagation.
17. What is explant?
18. Distinguish between Ti and Ri plasmid.
19. Define molecular probe.
20. What is single cell protein?

PART B                       ( 5 x 8 = 40 marks)

 

Answer any five, each within 350 words only. Draw diagrams and flowcharts wherever necessary.

21. Briefly explain the various sterilization techniques in plant tissue culture.
22. What is acclimatization? Explain how in vitro regenerated plants are transferred to

natural condition.

23. Define callus. Explain the types of callus. Add a note on its significance.
24. Define: a. Organogenesis b. Encapsulation of embryo
25. Cybrid d. Dedifferentiation.
26. Explain briefly the molecular interaction between Rhizobium and legume plants.
27. What is Taq DNA polymerase? Write down the steps involved in PCR.
28. Describe any two methods of direct gene transformation in plants.
29. Write notes on phycocolloids.

 

PART C                       ( 2 x 20 = 40 marks)

 

Answer the following, each within 1500  words only. Draw diagrams and flowcharts wherever necessary.

 

29a. Mention the significance of plant tissue culture techniques in plant sciences.

 

Or

1. Give an account of somatic hybridization. Add a note on its significance of

cybrids.

 

30a. i. Describe the structure and function of mitochondrial genome.

1. Explain the method of southern blotting.

Or

1. i. Explain how Ti plasmid is exploited in plant biotechnology.
2. Explain the role of yeast in gene cloning.

 

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