LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.
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FIFTH SEMESTER – April 2009
PB 5406 – IMMUNOLOGY AND IMMUNO TECHNOLOGY
Date & Time: 30/04/2009 / 1:00 – 4:00 Dept. No. Max. : 100 Marks
PART A (20 marks)
- Choose the correct answer (5 x 1 = 5)
- Edward Jenner is associated with vaccines against
- a) Rabies b) Chicken pox c) Small pox d) Hepatitis
- Light chain has _____ amino acids
- a) 110 b) 220 c) 100 d) 330
- The enzyme used in ELISA is
- a) protease b) lipase c) peroxidase d) amylase
- Which of the immunoglobin passes from mother into the foetus?
- a) Ig A b) Ig G c) Ig M d) Ig E
- Hybridoma technology was invented by
- a) Kohler b) Hanstein c) Pasteur d) Lister
- State whether the following statements are true or false (5 x 1 = 5)
- Pasteur invented Rabies vaccine.
- Ig D is a pentamer.
- Precipitaion occurs with soluble antigens.
- Western blotting is used in HIV detection.
- In Monoclonal antibody production, Myeloma cells are used because they are immortal.
III. Complete the following (5 x 1 = 5 )
- Vaccine is derived from a Greek word which means ______.
- The immunoglobin IgE is associated with __________.
- The principle of blood grouping is _______ .
- The technique that involves transfer of proteins to membrane is ________
- On activation, the B cells becomes
- Answer the following, each in about 50 words (5 x 1= 5)
- Mention the contributions of Koch to the field of immunology.
- What is a paratope?
- Define zone of equivalence.
- Define agglutination.
- What are haemetopoietic stem cells?
PART B
- Answer any five of the following, each answer not exceeding 350 words ( 5 x 8= 40)
- Mention the important milestones in immunology.
- Write notes on antigens.
- Discuss about national immunity schedule for newborn.
- Give an account of radioimmunoassay.
- Discuss briefly about immunoelectrophoresis.
- Discuss briefly about T lymphocytes.
- Explain about polyclonal antibodies and mention how are they produced?
- Write notes on humoral immunity.
PART C
- Answer the following, each answer not exceeding 1500 words.
(2 x 20 = 40)
- Write detailed notes on antibodies, their properties and structure
(or)
- Give a detailed account of vaccines and their types.
- Explain the process involved in Hybridoma technology
(or)
- Give a detailed account of Cell mediated immunity.
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