LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.

SIXTH SEMESTER – APRIL 2011

PB 6609/PB 6606 – FERMENTATION TECHNOLOGY

 

 

 

Date : 09-04-2011              Dept. No.                                        Max. : 100 Marks

Time : 9:00 – 12:00

 PART – A( 20 marks)

  ANSWER ALL THE QUESTIONS                                                                                                 

I.Choose the correct answer                                                                                                       (5×1=5)

1. Amylases are produced by
2. a) Aspergillus niger b) Bacillus licheniformis     c) Rhizopus       d) Phanerochaete

2.Air is supplied to a fermenter by

1. a) Sparger b) Baffles               c) Impeller                      d) Shaft
2. The preservation of cultures using freeze drying is called as
3. a) Sterilisation b) Ionisation          c) Lyophilisation          d) Tyndallisation
4. Antifoam agents used in industrial fermentations are
5. a) Silicones b) Esters           c) Alcohols                                 d) All of these
6. Cell disruption technique involves
7. a) Centrifugation b) Precipitation           c) Filtration                    d) Liquid shear

 

1. State whether the following statements are True or False           (5×1=5)
2. Secondary metabolites are produced during idiophase.
3. Batch culture is a closed culture containing limited amount of nutrients and inoculum.
4. Impellers prevent swirling and vortexing of the fluid.
5. Precipitationprocess is normally adopted for proteins.
6. Tachometers are sensors which penetrate into the interior of the fermenter.

III. Complete the following                                                                                                         (5×1=5)

11. An example of a product produced by a genetically engineered organism is __________.
12. Air bubbles are distributed uniformly in the fermenter by___________ .
13. The instrument used for measuring gas flow is ___________.
14. The cheapest source of carbon available from plant source is_____________.
15. The surfactant used in foam separation is________.

 

1. Answer the following each in 50 words           (5×1=5)
2. What are microbial metabolites?
3. What are the components in a synthetic media?
4. How will you adjust pH in a media?
5. How will you control foam?

20.Comment on filtration in downstream processing.

 

                                                                 PART-B                                                             

Answer any FIVE of the following questions in about 350 words.                 (5×7=35marks)     

 Draw necessary Diagrams.

21. a) Trace the development of industrial Biotechnology.

(OR)

1. b) Explain microbial
2. a) Write notes on the sterilization of media and equipment.

(OR)

1. b) Discuss microbial growth kinetics in a batch culture.
2. a) Describe the design of Airlift fermenter.

(OR)

1. b) Describe the aeration and agitation devices attached to a fermenter.
2. a) Discuss the methods of measuring dissolved oxygen.

(OR)

1. b) Write notes on online control of fermenters.
2. a) Comment on effluent treatment of industrial waste.

(OR)

1. b) Explain the technique of Centrifugation in downstream processing.

 

PART – C

Answer any THREE of the following not exceeding 1200 words.                  (3×15=45 marks)

Draw diagrams wherever necessary.

 

26. Explain the production and applications of microbial enzymes.
27. Explain in detail the operation of a continuous culture fermenter and how does it differ from

a Batch fermenter.

28. Describe the methods of preservation of industrially important microorganisms.
29. Discuss the role of Biosensors in the fermentation industry.
30. Describe the important processes in downstream processing.

 

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