LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & BIOTECHNOLOGY

AE 12

FIFTH SEMESTER – NOV 2006

         PB 5504 – PLANT BIOTECHNOLOGY

(Also equivalent to PBB 514)

 

 

Date & Time : 30-10-2006/9.00-12.00         Dept. No.                                                       Max. : 100 Marks

 

 

 

 

Part A                                           (20 marks)

Answer All questions

I Choose the Correct Answer                                                                     ( 5 x 1 = 5 marks)

 

01.The reversion of mature state of plant cells into meristamatic cells (callus) under

     in vitro condition is called …..

1. Differentiation b. Dedifferentiation    c. Redifferentiation     d. Totipotency

 

2. Which of the following disinfectant is used in explant sterilization?
3. Mercuric chloride b. Sodium hydroxide
4. Tween d. Ammonia
5. pBR 322 is constructed from ………..
6. Yeast             b. E.coli           c. Rhizobium               d. Bacteriophage
7. The gene for nodulin protein is encoded in ………
8. nod gene b. nif gene       c. plant genome           d. both a and c.
9. ……….. is used as stabilizers in ice cream.
10. Sodium alginate b. Carageneen                         c. Agar            d. Sodium nitrate

 

II State whether the following statements are True or False                (5 x 1 = 5 marks)

6. Autoclave is used to sterilize explants.
7. Embryo culture technique is used to break the seed dormancy.
8. Opines act as carbon and nitrogen source for the plants.
9. CaMV has double stranded DNA.
10. Cyanobacterium is commonly known as aquatic fern.

 

III. Complete the following                                                                     ( 5 x 1 = 5 marks)

11. ………… is used to convert haploid into diploid plant.
12. …….. is used to identify the regeneration of cell wall under in vitro condition.
13. The phenolic compound synthesized by Agrobacterium infected plant is ……
14. E. CoR₁ cut DNA at ………. end.
15. Spirulina contains ……… % of protein.

 

 

 

 

 

1. Answer all, each in about 50 words ( 5 x 1 = 5 marks)
2. Define somaclones.
3. What do you mean by encapsulation of embryo?
4. Distinguish between Ti plasmid and Ri plasmid.
5. Define Genomic library.
6. Mention the alga, which is used in cosmetics.

PART B                       (5 x 8 = 40 marks)

Answer any five, each within 350 words only. Draw diagrams and flowcharts wherever necessary.

21. Explain the basic steps in plant tissue culture.
22. Discuss in brief the biotechnological applications of plant cell and tissue culture in

plant sciences.

23. Expand the following with a footnote
24. DMSO b. PEG                        c. SDS-PAGE             d. HEPA
25. pUC f. IBA             g. TEMED                  h. BAP
26. Discuss the potentials of Ti plasmid of Agrobacterium tumefaciens in the genetic

engineering of plants.

25. With the suitable diagram, describe electrophoresis.
26. Describe the physical methods of gene transformation in plants.
27. Define phycocolloids. Enumerate the significance of phycocolloids.
28. What is phycoculture? Describe any two methods of phycoculture.

PART C                       ( 2 x 20 = 40 marks)

Answer the following, each within 1500 words only. Draw diagrams and flowcharts wherever necessary.

 

29. a). i) What are somaclonal variations? (5 marks)
30. ii) Write down the steps involved in cryopreservation.(7 marks)

iii) How do you produce artificial seeds under in vitro condition? (8 marks)

Or

b). i) Define totipotency. Explain the concept of totipotency and its exploitation in

Biotechnology.( 12 marks)

1. ii) Discuss the role of plant growth regulators in plant tissue culture. (8 marks)
2. “Chloroplast and Mitochondria are called as semiautonomous organelles” – Justify.

Or

Define Genetic Engineering. Elaborate on genetic engineering for insect resistance.

 

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