LOYOLA COLLEGE (AUTONOMOUS), CHENNAI– 600 034
M.Sc. DEGREE EXAMINATION -medical laboratory technology
FIRST SEMESTER – NOVEMBER 2003
ML 1805 clinical diaynostic microbiology
11.11.2003 Max: 100 Marks
1.00 – 4.00
SECTION – A
Answer ALL the questions (10 ´ 2 = 20 Marks)
- What is the function of capsules and slime layers?
- Define toxins and how is their potency expressed?
- What are differential stains?
- What is metachromasia?
- What are the different kinds of media?
- What is an enrichment medium?
- Define sterilisation.
- What are bactericidal and bacteriostatic agents?
- What is sanitization?
- Define virulence.
SECTION – B
Answer any four of the following (4 ´ 10 = 40 Marks)
- Describe the composition and structure of peptidoglycan, gram positive
and gram negative cell walls.
- Explain the structure of flagella and its distribution patterns.
- What are the three types of nutrient broth and how will you prepare them?
- What are disinfectants? What are the various applications of disinfectants?
- Discuss the spread and outbreak of infections in a community.
- Explain the infective hazards of intravenous infusion therapy.
SECTION – C
Answer any two of the following. (2 ´ 20 = 40 Marks)
- Define bacteraemia and explain on gram positive and gram
negative bacteraemia.
- Discuss in detail the special staining methods of flagella,
capsule, spores and granules with suitable diagrams.
- List the various physical methods of sterilisation and explain sterilisation by heat.
- Give an account on control and prevention of hospital acquired infections.
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