LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

   B.Sc. DEGREE EXAMINATION – CHEMISTRY & ADV. ZOOLOGY

THIRD SEMESTER – NOVEMBER 2010

    PB 3206 / 3200 – GENERAL MICROBIOLOGY

 

 

 

Date : 12-11-10                     Dept. No.                                        Max. : 100 Marks

Time : 9:00 – 12:00

PART A (20 marks)

 

Answer all questions.

 

1. Choose the correct answer: (5 x 1 = 5)

 

1. Which of the following is an example for brown algae?
2. a) Chlorella b) Chlamydomonas     c) Ectocarpus              d) Polysiphonia
3. Pili represent
4. Extrachromosomal genetic element   b)  protoplasmic outgrowth of donor cells
5. c) small flagella   d)  special bacterial cilia
6. High frequency recombination cells arise when
7. a) they have multiple F plasmids
8. b) F plasmid has been incorporated into the bacterial chromosome
9. c) conjugation and transformation happen at the same time
10. d) bacterial chromosomes do not break during conjugation
11. Name the pathogen that cause little leaf of brinjal:
12. a) Pseudomonas b) TMV                        c) CMV                                                d) Mycoplasma
13. Wastewater is characterized by a very low value of
14. a) turbidity b) BOD                        c) dissolved oxygen                 d) dissolved organics

 

1. State whether the following statements are true or false: (5 x 1 = 5)

 

6. Fungal cell wall is made of chitin.
7. Nutrient Agar medium is suitable for the cultivation of heterotrophic bacteria.
8. In transformation, the genetic material is transferred through bacteriophage.
9. Xanthomonas citri is a gram positive rod shaped bacterium.
10. Spoilage of meat by microbes can be prevented by storage at -20°C.

 

III. Complete the following:                                                                                                                   ( 5 x 1  = 5 )

 

11. The reserve food material in red algae is ________.
12. Bacteria which grow at very low temperatures are called _______.
13. Transfer of genetic material between cells by physical contact is called _______.
14. The host where the uredinal stage of Puccinia is seen is __________.
15. The organism used in the production of wine is _____________.

 

 

 

1. Answer the following, each in about 50 words: (5 x 1= 5)

 

16. What are complex viruses?
17. Define organolithotrophs.
18. What are Okazaki fragments?
19. Give the composition of Bordeaux mixture.
20. What is pasteurization?

 

PART  B

 

Answer the following, each answer not exceeding 350 words. Draw diagrams wherever necessary:                                                                                                                                                                           (5 x 7= 35)

 

21. List out the salient features of microalgae.

(or)

1. Give an account of the characteristic features of fungi.
2. Explain briefly about the development of endospore.

(or)

1. Write notes on the methods used for quantification of microorganisms.
2. Discuss briefly about the process of conjugation.

(or)

1. Give an account of gene regulation in bacteria.
2. Write short notes on citrus canker and its control.

(or)

1. Explain briefly about Tobacco mosaic disease.
2. Give an account of Cheese production.

(or)

1. Write short notes on microbial enzymes.

 

PART C

 

Answer any three of the following, each answer not exceeding 1200 words. Draw diagrams wherever necessary:                                                                                                                                                      (3 x 15= 45)

 

26. Discuss in detail about the salient features of viruses and their classification.
27. Write detailed notes on the ultrastructure of a bacterial cell.
28. Give a detailed account of a) transformation and b) transduction.
29. Explain about the symptoms, pathogen, disease cycle and control of Black rust disease in wheat.
30. Explain in detail about the processes involved in the treatment of domestic wastewater.

 

 

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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

B.Sc. DEGREE EXAMINATION – ADV. ZOOLOGY & CHEMISTRY

THIRD SEMESTER – NOVEMBER 2012

PB 3206 – GENERAL MICROBIOLOGY

 

 

Date : 09/11/2012             Dept. No.                                        Max. : 100 Marks

Time : 9:00 – 12:00

 

Part – A

 

Answer the following, each within 50 words:                                                                     (10 x 2 = 20)

 

1. Distinguish between Gram positive and Gram negative bacteria.
2. Write a short note on Biotecton.
3. What are pili?
4. What is DHA? Write a note on DHA.
5. What are the chemical components of bacterial cell wall?
6. Give the types of nutrient media for microbial culture.
7. What are fimbriae?
8. Define gene expression.
9. Mention the pathogens of Citrus canker and rust of Wheat.
10. What are vaccines?

 

Part – B

Answer the following, each within 500 words. Draw diagrams wherever necessary:           (5 x 7 = 35)

 

11(a). Briefly describe the phenotypic classification system of bacteria.

(OR)

(b). Enumerate the physical properties of viruses.

 

12(a). Describe Carbon and Sulphur cycles.

(OR)

(b). Briefly explain the biological treatment of sewage water.

 

13(a). Give the structure and function of flagella in microbes.

(OR)

(b). What are pure cultures of microbes? Provide the methods of their maintenance.

 

14(a). Briefly describe conjugation in bacteria.

(OR)

(b). Give a short account on little leaf disease of brinjal.

 

15(a). Discuss the types of microbial food spoilage and the methods of food preservation.

(OR)

(b). Enumerate the microbial enzymes with their commercial applications.

 

 

 

 

Part – C

Answer any three of the following, each within 1200 words. Draw diagrams wherever necessary:                                                                                                                                                                                                                               (3 x 15 = 45)

 

16. Elaborately discuss the importance of microalgae in changing the science fields.

 

17. Explain the physical and chemical processes of sewage water treatment.

 

18. Describe microbial growth in batch culture. Add a note on the methods of its measurement.

 

19. Write the pathogen, symptoms and control measures of Tobacco Mosaic Viral disease.

 

20. Define transduction. Describe in detail specialized transduction.

 

 

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