LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

M.Sc. DEGREE EXAMINATION – MEDICAL LAB TECHNOLOGY

HZ 5

SECOND SEMESTER – APRIL 2006

                                               ML 2952 – ADVANCED LAB. TECHNIQUES

 

 

Date & Time : 24-04-2006/9.00-12.00         Dept. No.                                                       Max. : 100 Marks

 

 

Part A                                     (Answer All)                          10 ´ 2 = 20

1. List out any four protein transfer procedure.
2. What is ponceou S and PVDF?
3. What are ramification amplification?
4. Write the goals of HGP.
5. Define gene therapy.
6. Define obligatory paternal gene.
7. What is direct exclusion?
8. Define Two haplotype exclusion
9. What is parentage testing?
10. Expand the abbreviations: SWGDAM, FBI, CODIS, NDIS.

Part B                                     (Answer any four)                 4 ´ 10 = 40

11. Write a short note on DNA polymorphism.
12. Describe in brief human leukocyte antigen.
13. Comment on documentation and reporting parentage-testing results.
14. Describe in detail any two gene sequencing method.
15. Give an account on human genome project.
16. Describe in detail the micro array procedure and add a note on its clinical application.

 

Part C                                     (Answer any two)                              2 ´ 20 = 40

17. Explain in detail the DNA testing method.
18. Describe molecular techniques in the diagnosis of hematopoitic neoplasm.
19. Write the importance of molecular techniques used in the diagnosis of leukemia.
20. Discuss the techniques to detect antigen receptor gene rearrangement.

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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

LM 10

M.Sc. DEGREE EXAMINATION – MEDICAL LAB. TECH.

SECOND SEMESTER – APRIL 2008

    ML 2952 – ADVANCED LAB. TECHNIQUES

 

 

 

Date : 24/04/2008            Dept. No.                                        Max. : 100 Marks

Time : 1:00 – 4:00

SECTION A

Answer ALL the questions:                                                            (10 x 2 = 20 Marks)

1. What are the advantages of nucleic acid based identification methods?
2. What is VNTR?
3. Mention the characteristics of the different types of restriction endonucleases.
4. What is homopolymer tailing?
5. Define homologous chromosome.
6. What is an allele?
7. Define mutation.
8. What is mosaicism?
9. Mention the uses of DNA in forensic science.
10. List the molecular techniques used in forensic investigation.

SECTION B

Answer any FOUR of the following                                             (4 x 10 = 40 Marks)

11. Write briefly on gel electrophoresis.
12. Give the difference between southern, northern and western blotting techniques.
13. Write a brief account on plasmids.
14. How is cell culture done for karyotyping? Explain.
15. Write short notes on Prader Willi syndrome and Angelman syndrome.
16. Give a brief account on DNA databases.

SECTION C

Answer any TWO of the following                                              (2 x 20 = 40 Marks)

17. Write in detail about PCR technique.
18. Explain in detail about the quality control and quality assurance in a molecular

diagnostic laboratory.

19. Write in detail on structural chromosomal abnormalities.
20. Explain in detail about Y chromosome analysis and Mitochondrial DNA analysis.

 

 

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        LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

M.Sc. DEGREE EXAMINATION – MEDICAL LAB. TECHNOLOGY

BV 10

SECOND SEMESTER – April 2009

ML 2954 / 2952 – ADVANCED LAB.TECHNIQUES

 

 

 

Date & Time: 24/04/2009 / 1:00 – 4:00   Dept. No.                                                    Max. : 100 Marks

 

 

SECTION A

Answer ALL the questions in one or two sentences:                                 (10 X 2 = 20 marks)

1. What is CODIS?
2. How can DNA be used to identify an individual?
3. List the factors affecting southern blotting.
4. What are the three steps in AmFLP?
5. Define allele.
6. What is a primer?
7. Draw the structure of a chromosome.
8. What abnormalities can be detected by cytogenetics?
9. Give examples of how DNA can be used in forensic identification.
10. What is STR analysis?

SECTION B

Answer any FOUR of the following:                                                          (4  X 10 = 40 marks)

11. What is Y chromosome analysis? How is it useful in genealogy?
12. Write short notes on RFLP.
13. Explain the methods of isolation and purification of nucleic acids.
14. Explain structural abnormalities of chromosomes with diagram.
15. Discuss briefly quality control and quality assurance in a molecular diagnostic laboratory.
16. Elaborate northern, southern and western blotting.

SECTION C

Answer any TWO of the following:                                                            (2 X 20 = 40 marks)

17. Discuss in detail chromosomes preparations for diagnosis.
18. How is DNA fingerprinting done? Explain.
19. Explain in detail the PCR technique. Add a note on its applications.
20. Write an essay on in situ

 

 

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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

M.Sc. DEGREE EXAMINATION – MEDICAL LAB. TECHNOLOGY

SECOND SEMESTER – APRIL 2012

ML 2954 – ADVANCED LAB.TECHNIQUES

 

 

Date : 21-04-2012             Dept. No.                                        Max. : 100 Marks

Time : 9:00 – 12:00

 

Answer ALL the questions:                    PART A                                                    10×2=20 marks

1. What is DNA chip technology?
2. Comment on contamination control in molecular lab.
3. List down DNA testing methods.
4. What are transgenic organisms?
5. Differentiate pre-amplification from post-amplification room.
6. What are cosmids?
7. Distinguish T4 bacteriophage.
8. Differentiate cell based from cell free DNA cloning.
9. Comment on gene mapping.
10. Define obligatory paternal gene.

Answer any FOUR:                                    PART B                                                    4×10=40 marks

11. Describe DNA polymorphism.
12. Explain the clinical applications of microarray.
13. Write notes on application of DNA in forensic science.
14. Give short account on western blotting technique.
15. Describe types of PCR.
16. Write notes on gene therapy.

Answer any TWO:                                      PART C                                                    2×20=40 marks

17. Give an account on educational and training requirements for personnel working in molecular laboratories.
18. Write an essay on electrophoretic separation.
19. Give an account on Stem cells.
20. Explain in detail the parentage testing.

 

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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

M.Sc. DEGREE EXAMINATION – MEDICAL LAB. TECHNOLOGY

SECOND SEMESTER – NOVEMBER 2012

ML 2954 – ADVANCED LAB.TECHNIQUES

 

 

Date : 10/11/2012            Dept. No.                                        Max. : 100 Marks

Time : 1:00 – 4:00

 

Answer ALL the questions:                                 PART A                                       2×10=20 marks

1. Enumerate the solid supports used in electrophoresis.
2. What is gene cloning?
3. What is DNA finger printing?
4. Define the role of restriction endonucleases.
5. Define FISH and FICTION.
6. What is paternity test?
7. Define fluorescent detection procedure.
8. Differentiate sticky ends from blunt ends.
9. What are transgenic animals?
10. Enumerate blocking buffers of western blotting technique.

Answer any FOUR:                                                PART B                                        4×10=40 marks

11. Write notes on DNA polymorphism.
12. Give an account on PAGE.
13. Describe northern blotting.
14. Give an account on Human Genome Project.
15. Explain clinical importance of ELISA.
16. Write notes on gene mapping.

Answer any TWO:                                                 PART C                                         2×20=40 marks

17. Write a detailed account on microarray. Add note on clinical applications.
18. Discuss polymerase chain reaction.
19. Describe in detail the significance of DNA over traditional serology.
20. Write an essay on hybridization.

 

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