LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION – MEDICAL LAB. TECH.
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FOURTH SEMESTER – APRIL 2007
ML 4807 – HISTOPATHOLOGY AND ESSENTIALS OF LAB
Date & Time: 18/04/2007 / 9:00 – 12:00 Dept. No. Max. : 100 Marks
SECTION-A
Answer all the Questions: (10 x 2 = 20 Marks)
- What are infiltration and impregnation?
- How can mineralized bone be softened for sectioning.
- What is PAP smear?
- What is meant by vacuum embedding?
- Define: a) Honing b) Stropping.
- Write the preparation and use of egg albumin.
- Mention different types of biopsy.
- What is amyloidosis?
- Comment on decalcification.
- Mention phenyl and Indole group of amino acids.
SECTION-B
Answer any four of the following: (4 x 10 = 40 Marks)
- Write a short note on mucopolysaccharides and DMAB staining.
- Give an account of cryostat sectioning.
- Explain the disulfide and sulfhydryl groups of amino acids.
- Write the principle, procedure and histological importance of methyl
green-pyronin method.
- What is exfoliative cytology? Explain preparation and fixation of cytological
specimen.
- Enumerate the problems encountered during section cutting.
SECTION-C
Answer any two of the following: ( 2 x 20 = 40 Marks)
- Write an essay on different types of fixatives.
- What is glycosaminoglycans? Explain the principle, procedure and histological
importance of PAS staining.
- Write short notes on : a) Nile blue sulfate stain.
- b) Freezing microtome.
- Describe in detail any three-lipid identification methods.