Loyola College Separation Techniques & Pharmaceutical Chemistry Question Papers
Loyola College M.Sc. Medical Lab Technology April 2011 Separation Techniques & Pharmaceutical Chemistry Question Paper PDF Download
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION – MEDICAL LAB. TECHNOLOGY
FOURTH SEMESTER – APRIL 2011
ML 4810 – SEPARATION TECHNIQUES & PHARMACEUTICAL CHEMISTRY
Date : 07-04-2011 Dept. No. Max. : 100 Marks
Time : 9:00 – 12:00
SECTION-A
ANSWER ALL THE QUESTIONS: (10 X 2=20 Marks)
- What is Svedberg unit?
- Differentiate rate-zonal from isopycnic centrifugation.
- What are isocratic and gradient elution?
- List any four locating agents used in TLC.
- Mention any two membranes used in blotting technique.
- What is isoelectric focussing?
- Name any four infectious agents which act as teratogens.
- What is Real Time PCR?
- Define the term bioenhancer.
- What is lethal and sublethal toxic effects?
SECTION-B
ANSWER ANY FOUR QUESTIONS (4 X 10=40 Marks)
- Explain the rate zonal and isopycnic centrifugation technique.
- Write notes on molecular exclusion chromatography.
- Write the principle, procedure and applications of western blotting technique.
- Give an account of the mechanism of affinity chromatograpy.
- Discuss the different routs of excretion of drug.
- Elaborate the impacts of heavy metal toxicity with suitable example.
SECTION-C
ANSWER ANY TWO QUESTIONS (2 X 20=40 Marks)
- Explain the mechanism, procedure and applications of preparative centrifugation.
- Describe the principle, operation and applications of SDS-PAGE.
- Trace the polymerization chain reaction with suitable diagram.
- Discuss the biotransformation of drugs.
Loyola College M.Sc. Medical Lab Technology April 2012 Separation Techniques & Pharmaceutical Chemistry Question Paper PDF Download
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION – MEDICAL LAB. TECHNOLOGY
FOURTH SEMESTER – APRIL 2012
ML 4810 – SEPARATION TECHNIQUES & PHARMACEUTICAL CHEMISTRY
Date : 18-04-2012 Dept. No. Max. : 100 Marks
Time : 1:00 – 4:00
PART-A
Answer ALL the questions: (10 X 2=20 Marks)
- What is sedimentation co-efficient?
- Define isoelectric focusing.
- Name the various locating agents used in TLC.
- What are gradient and isocratic elution?
- Write down the importance of protease and RNase in DNA isolation.
- List the enteral routes of drug administration.
- Mention the membranes used in blotting techniques.
- Distinguish teratogens from mutagens.
- Define electrophoretic mobility.
- What is probe hybridization?
PART-B
Answer any FOUR (4 X 10 =40 Marks)
- Classify and write the basic principle of centrifugation and add a note on rotors.
- What is meant by blotting? Explain southern blotting technique.
- Write notes on affinity chromatography with suitable diagram.
- Describe the steps involved in isolation and separation of RNA.
- Elaborate the mechanism of drug absorption and distribution.
- What are acute and chronic toxicities? Write notes on heavy metal toxicity.
PART-C
Answer any TWO (2 X 20=40 Marks)
- Explain the mechanism and application of preparative centrifugation.
- What is zwitterion? Describe SDS-PAGE electrophoresis.
- Elaborate the principle, procedure and diagnostic importance of PCR techniques.
- Discuss general toxicology and toxicokinetics.
