M.Sc Medical Lab Technology Advanced Lab.Techniques Question Paper 2012

Loyola College M.Sc. Medical Lab Technology April 2012 Advanced Lab.Techniques Question Paper PDF Download

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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

M.Sc. DEGREE EXAMINATION – MEDICAL LAB. TECHNOLOGY

SECOND SEMESTER – APRIL 2012

ML 2954 – ADVANCED LAB.TECHNIQUES

 

 

Date : 21-04-2012             Dept. No.                                        Max. : 100 Marks

Time : 9:00 – 12:00

 

Answer ALL the questions:                    PART A                                                    10×2=20 marks

  1. What is DNA chip technology?
  2. Comment on contamination control in molecular lab.
  3. List down DNA testing methods.
  4. What are transgenic organisms?
  5. Differentiate pre-amplification from post-amplification room.
  6. What are cosmids?
  7. Distinguish T4 bacteriophage.
  8. Differentiate cell based from cell free DNA cloning.
  9. Comment on gene mapping.
  10. Define obligatory paternal gene.

Answer any FOUR:                                    PART B                                                    4×10=40 marks

  1. Describe DNA polymorphism.
  2. Explain the clinical applications of microarray.
  3. Write notes on application of DNA in forensic science.
  4. Give short account on western blotting technique.
  5. Describe types of PCR.
  6. Write notes on gene therapy.

Answer any TWO:                                      PART C                                                    2×20=40 marks

  1. Give an account on educational and training requirements for personnel working in molecular laboratories.
  2. Write an essay on electrophoretic separation.
  3. Give an account on Stem cells.
  4. Explain in detail the parentage testing.

 

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Loyola College M.Sc. Medical Lab Technology Nov 2012 Advanced Lab.Techniques Question Paper PDF Download

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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

M.Sc. DEGREE EXAMINATION – MEDICAL LAB. TECHNOLOGY

SECOND SEMESTER – NOVEMBER 2012

ML 2954 – ADVANCED LAB.TECHNIQUES

 

 

Date : 10/11/2012            Dept. No.                                        Max. : 100 Marks

Time : 1:00 – 4:00

 

Answer ALL the questions:                                 PART A                                       2×10=20 marks

  1. Enumerate the solid supports used in electrophoresis.
  2. What is gene cloning?
  3. What is DNA finger printing?
  4. Define the role of restriction endonucleases.
  5. Define FISH and FICTION.
  6. What is paternity test?
  7. Define fluorescent detection procedure.
  8. Differentiate sticky ends from blunt ends.
  9. What are transgenic animals?
  10. Enumerate blocking buffers of western blotting technique.

Answer any FOUR:                                                PART B                                        4×10=40 marks

  1. Write notes on DNA polymorphism.
  2. Give an account on PAGE.
  3. Describe northern blotting.
  4. Give an account on Human Genome Project.
  5. Explain clinical importance of ELISA.
  6. Write notes on gene mapping.

Answer any TWO:                                                 PART C                                         2×20=40 marks

  1. Write a detailed account on microarray. Add note on clinical applications.
  2. Discuss polymerase chain reaction.
  3. Describe in detail the significance of DNA over traditional serology.
  4. Write an essay on hybridization.

 

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