LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION – MEDICAL LAB. TECHNOLOGY
FOURTH SEMESTER – APRIL 2012
ML 4810 – SEPARATION TECHNIQUES & PHARMACEUTICAL CHEMISTRY
Date : 18-04-2012 Dept. No. Max. : 100 Marks
Time : 1:00 – 4:00
PART-A
Answer ALL the questions: (10 X 2=20 Marks)
- What is sedimentation co-efficient?
- Define isoelectric focusing.
- Name the various locating agents used in TLC.
- What are gradient and isocratic elution?
- Write down the importance of protease and RNase in DNA isolation.
- List the enteral routes of drug administration.
- Mention the membranes used in blotting techniques.
- Distinguish teratogens from mutagens.
- Define electrophoretic mobility.
- What is probe hybridization?
PART-B
Answer any FOUR (4 X 10 =40 Marks)
- Classify and write the basic principle of centrifugation and add a note on rotors.
- What is meant by blotting? Explain southern blotting technique.
- Write notes on affinity chromatography with suitable diagram.
- Describe the steps involved in isolation and separation of RNA.
- Elaborate the mechanism of drug absorption and distribution.
- What are acute and chronic toxicities? Write notes on heavy metal toxicity.
PART-C
Answer any TWO (2 X 20=40 Marks)
- Explain the mechanism and application of preparative centrifugation.
- What is zwitterion? Describe SDS-PAGE electrophoresis.
- Elaborate the principle, procedure and diagnostic importance of PCR techniques.
- Discuss general toxicology and toxicokinetics.