LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

M.Sc. DEGREE EXAMINATION – ZOOLOGY

FOURTH SEMESTER – APRIL 2012

ZO 4811 – BIOTECHNOLOGY

 

 

Date : 20-04-2012             Dept. No.                                        Max. : 100 Marks

Time : 1:00 – 4:00

 

SECTION – A

Answer ALL questions:                                                                                                             (10×2= 20)

 

1. Distinguish event specific from construct specific detection of GMO.
2. Name any four restriction enzymes.
3. Sketch PBR 322
4. Comment on golden rice and azolla.
5. Define nutrigenomics.
6. State the significance of knockout mice and suicide gene therapy.
7. Distinguish morphological markers from biochemical markers.
8. What do you know about Integria?
9. Define downstream processing
10. Expand: a) DMSO    b) WIPO

SECTION – B

Answer any FOUR questions  briefly:                                                                                      (4×10= 40)

 

11. How do you create transgenic animals using the provision of microinjection

and embryonic stem cells?

12. Draft a protocol for cryopreservation and add its applications.
13. How do you immobilize enzymes? Mention its significance.
14. State the properties of restriction recognition sites
15. Illustrate direct and cell based delivery system towards gene therapy.
16. What are the challenges in DNA computing?

 

SECTION – C

Answer any TWO questions in detail:                                                                                     (2×20= 40)

 

17. Illustrate nucleic acid hybridization and DNA finger printing techniques.

 

18. Describe any four methods of cloning with vector

 

19. Propose a design of conventional bioreactor with all their components and

explain its implications in bioprocessing.

 

20. Highlight on : a) Risks of IVF  and infertility therapy and
21. b) Risks of Biotechniques.

 

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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

M.Sc. DEGREE EXAMINATION – ZOOLOGY

FOURTH SEMESTER – NOVEMBER 2012

ZO 4811 – BIOTECHNOLOGY

 

 

Date : 06/11/2012            Dept. No.                                        Max. : 100 Marks

Time : 1:00 – 4:00

SECTION – A

Answer ALL questions:                                                                                                        (10×2= 20)

 

1. Distinguish bioleaching from bioremediation.
2. Name the filter paper being used in southern blotting and northern blotting.
3. State Wallac rule on melting temperature.
4. Sketch Ti Plasmid.
5. Distinguish monoclonal from polyclonal antibodies.
6. What are molecular markers? Name any two of them.
7. What are base cutters? Mention the types.
8. What are magic bullets? State the importance.
9. What are shuttle vectors? Mention their application.
10. Expand: a) PCB b) HRCS

 

 

SECTION – B

 

Answer any FOUR of the following briefly:                                                                         (4×10= 40)

 

11. How do microbes oxidize Fe₂⁺ into Fe₃⁺ ? Mention its significance?
12. What are the criticisms raised against GMO?
13. “Heart of fermentation is bioreactor” – Substantiate.
14. What are the significant decisions taken by Govt of India regarding patent

system?

15. What are the risks and ethics involved in gene therapy?
16. Describe any two cloning vectors.

 

SECTION – C

 

Answer any TWO of the following in detail:                                                                         (2×20=40)

 

17. How do you resort to animal cell culture and organ culture? What are their applications?
18. What are the strategies and tools involved in DNA computing?
19. Elucidate the strategies involved in large scale production of enzymes.
20. Discuss the impact of biotechnology on Agricultural crops.

 

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